NRF-1 polyclonal antibody 
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Product Name :
NRF-1 polyclonal antibody Background :
Nuclear respiratory factor-1 (NRF-1) is a transcriptional activator that has been implicated in the nuclear control of respiratory chain expression in mammalian cells. The NRF-1 gene is expressed during oogenesis and during the early stages of embryogenesis. In vitro studies have implicated NRF-1 in the transcriptional expression of nuclear genes required for mitochondrial respiratory function, as well as for other fundamental cellular activities. While most isolated wild-type and NRF-1+/- blastocysts continue to develop normally in vitro, NRF-1-/- blastocysts lack this ability, despite their normal morphology. NRF-1 is specifically required in the maintenance of mtDNA and respiratory chain function during early embryogenesis. NRF-1 also plays a key role in cellular adaptation to energy demands by translating physiological signals into an increased capacity for generating energy. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NRF-1 polyclonal antibody detects endogenous levels of NRF-1 protein. Immunogen :
Recombinant full length Human NRF-1. Conjugate :
Unconjugated Modification :
Unmodified
NRF-1 polyclonal antibody Background :
Nuclear respiratory factor-1 (NRF-1) is a transcriptional activator that has been implicated in the nuclear control of respiratory chain expression in mammalian cells. The NRF-1 gene is expressed during oogenesis and during the early stages of embryogenesis. In vitro studies have implicated NRF-1 in the transcriptional expression of nuclear genes required for mitochondrial respiratory function, as well as for other fundamental cellular activities. While most isolated wild-type and NRF-1+/- blastocysts continue to develop normally in vitro, NRF-1-/- blastocysts lack this ability, despite their normal morphology. NRF-1 is specifically required in the maintenance of mtDNA and respiratory chain function during early embryogenesis. NRF-1 also plays a key role in cellular adaptation to energy demands by translating physiological signals into an increased capacity for generating energy. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NRF-1 polyclonal antibody detects endogenous levels of NRF-1 protein. Immunogen :
Recombinant full length Human NRF-1. Conjugate :
Unconjugated Modification :
Unmodified
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Western blot analysis of NRF1 expression in MCF7 (A), mouse testis (B) whole cell lysates.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.