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Bcl-2 Polyclonal Antibody BS65629
  • Western blot analysis of BCL2 expression in H9C2 (A), CT26 (B), MCF7 (C), HepG2 (D) whole cell lysates.
  • Immunohistochemical analysis of BCL2 staining in human brain formalin fixed paraffin embedded tissue section.
  • Immunohistochemical analysis of BCL2 staining in human brain formalin fixed paraffin embedded tissue section.
Product NameBcl-2 Polyclonal Antibody
Catalog No.BS65629
Swiss-ProtP10415
Host Rabbit
ReactivityHuman, Mouse, Rat,
ApplicationsWB, IHC, IF/ICC
Application_allWB,1:500 - 1:1000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200
BiowMW~ 28 kDa
Alternative NameApoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN.
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $208
100ul $358
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Product Name :
Bcl-2 Polyclonal Antibody
Background :
The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14;18)-bearing follicular B cell lymphomas(1,2).Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3,5).Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action (6,7).A more recently identified protein,designated Bax p21(i.e., Bcl-associated X protein ),has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2(8). Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2(8).
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Bcl-2 Polyclonal Antibody detects endogenous levels of Bcl-2 protein.
Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BCL2.
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of BCL2 expression in H9C2 (A), CT26 (B), MCF7 (C), HepG2 (D) whole cell lysates.
  • Immunohistochemical analysis of BCL2 staining in human brain formalin fixed paraffin embedded tissue section.
  • Immunohistochemical analysis of BCL2 staining in human brain formalin fixed paraffin embedded tissue section.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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