CREB (phospho-S133) polyclonal antibody 
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MSDS
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Product Name :
CREB (phospho-S133) polyclonal antibody Background :
Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-CREB (S133) polyclonal antibody detects endogenous levels of CREB-1 protein only when phosphorylated at Ser133. Immunogen :
Synthetic phosphopeptide derived from human CREB around the phosphorylation site of Serine 133. Conjugate :
Unconjugated Modification :
Phosphorylated
CREB (phospho-S133) polyclonal antibody Background :
Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-CREB (S133) polyclonal antibody detects endogenous levels of CREB-1 protein only when phosphorylated at Ser133. Immunogen :
Synthetic phosphopeptide derived from human CREB around the phosphorylation site of Serine 133. Conjugate :
Unconjugated Modification :
Phosphorylated
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Western blot analysis of CREB (Phospho-S133) expression in C6 (A), NIH3T3 (B), HEK293T (C), H1792 (D) whole cell lysates. -
Immunohistochemical analysis of CREB (Phospho-S133) staining in human breast cancer formalin fixed paraffin embedded tissue section.
The novel squamosamide derivative FLZ enhances BDNF/TrkB/CREB signaling and inhibits neuronal apoptosis in APP/PS1 mice
PMCID: Pubmed No.:20154710
SHAFTS: A Hybrid Approach for 3D Molecular Similarity Calculation. 2. Prospective Case Study in the Discovery of Diverse p90 Ribosomal S6 Protein Kinase 2 Inhibitors To Suppress Cell Migration
PMCID: Pubmed No.:21488662
Norisoboldine inhibits the production of interleukin-6 in fibroblast-like synoviocytes from adjuvant arthritis rats through PKC/MAPK/NF-κB-p65/CREB pathways
PMCID: Pubmed No.:22473817
Picroside II protects cardiomyocytes from hypoxia/reoxygenation-induced apoptosis by activating the PI3K/Akt and CREB pathways
PMCID: Pubmed No.:22581361
Effects of Nicorandil in Neuroprotective Activation of PI3K/AKT Pathways in a Cellular Model of Alzheimer's Disease
PMCID: Pubmed No.:24008440
UCH-L1 Inhibition Involved in CREB Dephosphorylation in Hippocampal Slices
PMCID: Pubmed No.:24323362
Prostacyclin Analogue Beraprost Inhibits Cardiac Fibroblast Proliferation Depending on Prostacyclin Receptor Activation through a TGF β-Smad Signal Pathway
PMCID: Pubmed No.:24852754
In vivo imaging of CREB phosphorylation in awake-mouse brain
PMCID: Pubmed No.:26044058
Exposure to bisphenol A (BPA) in Wistar rats reduces sperm quality with disruption of ERK signal pathway
PMCID: Pubmed No.:26862991
miR-133b reverses the hydrosalpinx-induced impairment of embryo attachment through down-regulation of SGK1
PMCID: Pubmed No.:26840046
Oridonin Attenuates Synaptic Loss and Cognitive Deficits in an Aβ 1–42-Induced Mouse Model of Alzheimer's Disease
PMCID: Pubmed No.:26974541
Exposure to bisphenol A (BPA) in Wistar rats reduces sperm quality with disruption of ERK signal pathway
PMCID: Pubmed No.:26862991
miR-133b reverses the hydrosalpinx-induced impairment of embryo attachment through down-regulation of SGK1
PMCID: Pubmed No.:26840046
Oridonin Attenuates Synaptic Loss and Cognitive Deficits in an Aβ 1–42-Induced Mouse Model of Alzheimer's Disease
PMCID: Pubmed No.:26974541
UCH-L1 Inhibition Involved in CREB Dephosphorylation in Hippocampal Slices
PMCID: Pubmed No.:24323362
Pretreatment with low dose etomidate prevents etomidate-induced rat adrenal insufficiency by regulating oxidative stress-related MAPKs and apoptosis
PMCID: Pubmed No.:25989534
ERα and/or ERβ activation ameliorates cognitive impairment, neurogenesis and apoptosis in type 2 diabetes mellitus mice
PMCID: Pubmed No.:30201537
Neuroprotective effects of novel compound Tozan on cognition, neurogenesis and apoptosis in diabetes
PMCID: Pubmed No.:34734023
Activation of ERα and/or ERβ ameliorates cognitive impairment and apoptosis in streptozotocin-induced diabetic mice
PMCID: Pubmed No.:30096284
Adipokine zinc-α2-glycoprotein alleviates lipopolysaccharide-induced inflammatory responses through the β3-AR/PKA/CREB pathway
PMCID: Pubmed No.:31260855
Neuroprotective effects of novel compound FMDB on cognition, neurogenesis and apoptosis in APP/PS1 transgenic mouse model of Alzheimer's disease
PMCID: Pubmed No.:36893915
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.