Product Name :
ERK1/2 (T202/Y204) polyclonal antibody Background :
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and Threonine sites mapping within a characteristic Thr- Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phos-phorylate downstream components on Serine and Threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ERK1/2 (T202/Y204) polyclonal antibody detects endogenous levels of ERK1/2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 180-220 of Human ERK1. Conjugate :
Unconjugated Modification :
Unmodified
ERK1/2 (T202/Y204) polyclonal antibody Background :
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and Threonine sites mapping within a characteristic Thr- Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phos-phorylate downstream components on Serine and Threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ERK1/2 (T202/Y204) polyclonal antibody detects endogenous levels of ERK1/2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 180-220 of Human ERK1. Conjugate :
Unconjugated Modification :
Unmodified
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Western blot (WB) analysis of ERK1/2 pAb at 1:500 dilution Lane1:U-87MG whole cell lysate(40ug) Lane2:H9C2 whole cell lysate(40ug) Lane3:EC9706 whole cell lysate(40ug)
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Western blot (WB) analysis of ERK1/2 (T202/Y204) pAb at 1:500 dillution Lane1:Hela whole cell lysate(20μg) Lane2:NIH-3T3 whole cell lysate(40μg) Lane3:H9C2 whole cell lysate(40μg)
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Western blot (WB) analysis of ERK1/2 (T202/Y204) pAb at 1:500 dillution Lane1:Hela whole cell lysate(20μg) Lane2:NIH-3T3 whole cell lysate(40μg) Lane3:H9C2 whole cell lysate(40μg)
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Western blot (WB) analysis of ERK1/2 (T202/Y204) pAb at 1:500 dillution Lane1:Hela whole cell lysate(20μg) Lane2:NIH-3T3 whole cell lysate(40μg) Lane3:H9C2 whole cell lysate(40μg)
Dose-dependent effect of tamoxifen in tamoxifen-resistant breast cancer cells via stimulation by the ERK1/2 and AKT signaling pathways.
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Dihydrotestosterone alters urocortin levels in human umbilical vein endothelial cells.
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Lipoxin A4-induced heme oxygenase-1 protects cardiomyocytes against hypoxia/reoxygenation injury via p38 MAPK activation and Nrf2/ARE complex.
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An apple oligogalactan suppresses endotoxin-induced cyclooxygenase-2 expression by inhibition of LPS pathways
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MicroRNA-200a regulates Grb2 and suppresses differentiation of mouse embryonic stem cells into endoderm and mesoderm.
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The stimulation of osteogenic differentiation of mesenchymal stem cells and vascular endothelial growth factor secretion of endothelial cells by β-CaSiO3/β-Ca3(PO4)2 scaffolds
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Grape Seed Procyanidin Reversal of P-glycoprotein Associated Multi-Drug Resistance via Down-regulation of NF-κB and MAPK/ERK Mediated YB-1 Activity in A2780/T Cells
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Self-assembling nanofibers improve cognitive impairment in a transgenic mice model of Alzheimer's disease
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Musashi2 modulates K562 leukemic cell proliferation and apoptosis involving the MAPK pathway
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ZFX knockdown inhibits growth and migration of non-small cell lung carcinoma cell line H1299
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Knockdown of protein tyrosine phosphatase receptor U inhibits growth and motility of gastric cancer cells
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Modulatory Effects of Vasoactive Intestinal Peptide on Intestinal Mucosal Immunity and Microbial Community of Weaned Piglets Challenged by an Enterotoxigenic Escherichia coli (K88).
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Expression of ERK and p-ERK proteins of ERK signaling pathway in the kidneys of fluoride-exposed carp (Cyprinus carpio).
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Ubiquitin‑specific protease 22‑induced autophagy is correlated with poor prognosis of pancreatic cancer
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Diosgenin inhibits superoxide generation in FMLP-activated mouse neutrophils via multiple pathways
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MSTN, mTOR and FoxO4 Are Involved in the Enhancement of Breast Muscle Growth by Methionine in Broilers with Lower Hatching Weight
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Role of the MAPK pathway in the observed bystander effect in lymphocytes co-cultured with macrophages irradiated with γ-rays or carbon ions
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Epigallocatechin-3-gallate attenuates lipopolysaccharide-induced mastitis in rats via suppressing MAPK mediated inflammatory responses and oxidative stress
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Inhibitory effects of Chikusetsusaponin IVa on lipopolysaccharide-induced pro-inflammatory responses in THP-1 cells.
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Fluid Shear Stress Inhibits TNF-α-induced Osteoblast Apoptosis via ERK5 Signaling Pathway
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8-Methoxypsoralen Induces Intrinsic Apoptosis in HepG2 Cells: Involvement of Reactive Oxygen Species Generation and ERK1/2 Pathway Inhibition
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Phloretin induces apoptosis of non-small cell lung carcinoma A549 cells via JNK1/2 and p38 MAPK pathways
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DGAEE, a newly synthesized derivative of glycyrrhetinic acid, potently attenuates mouse septic shock via its main metabolite DGA in an IL-10-dependent manner
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A novel acetylcholinesterase inhibitor and calcium channel blocker SCR-1693 improves Aβ25–35-impaired mouse cognitive function
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Lipoxin A4 Attenuates Cell Invasion by Inhibiting ROS/ERK/MMP Pathway in Pancreatic Cancer
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Overexpression of miR-335 confers cell proliferation and tumour growth to colorectal carcinoma cells
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Dioscin suppresses human laryngeal cancer cells growth via induction of cell-cycle arrest and MAPK-mediated mitochondrial-derived apoptosis and inhibition of tumor invasion.
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MicroRNA-9 regulates cardiac fibrosis by targeting PDGFR-β in rats
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Dynamic monitoring of GPER-mediated estrogenic effects in breast cancer associated fibroblasts: an alternative role of estrogen in mammary carcinoma development
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TNF‐α downregulates CIDEC via MEK/ERK pathway in human adipocytes
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Fluid shear stress suppresses TNF-α-induced apoptosis in MC3T3-E1 cells: Involvement of ERK5-AKT-FoxO3a-Bim/FasL signaling pathways
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Knockdown of protein tyrosine phosphatase receptor U inhibits growth and motility of gastric cancer cells
PMCID: Pubmed No.:25337216
Modulatory Effects of Vasoactive Intestinal Peptide on Intestinal Mucosal Immunity and Microbial Community of Weaned Piglets Challenged by an Enterotoxigenic Escherichia coli (K88).
PMCID: Pubmed No.:25101851
Expression of ERK and p-ERK proteins of ERK signaling pathway in the kidneys of fluoride-exposed carp (Cyprinus carpio).
PMCID: Pubmed No.:25190107
Ubiquitin‑specific protease 22‑induced autophagy is correlated with poor prognosis of pancreatic cancer
PMCID: Pubmed No.:25241857
Diosgenin inhibits superoxide generation in FMLP-activated mouse neutrophils via multiple pathways
PMCID: Pubmed No.:25246240
MSTN, mTOR and FoxO4 Are Involved in the Enhancement of Breast Muscle Growth by Methionine in Broilers with Lower Hatching Weight
PMCID: Pubmed No.:25437444
Self-assembling nanofibers improve cognitive impairment in a transgenic mice model of Alzheimer's disease
PMCID: Pubmed No.:24103374
Musashi2 modulates K562 leukemic cell proliferation and apoptosis involving the MAPK pathway
PMCID: Pubmed No.:24076374
ZFX knockdown inhibits growth and migration of non-small cell lung carcinoma cell line H1299
PMCID: Pubmed No.:24228108
Phloretin induces apoptosis of non-small cell lung carcinoma A549 cells via JNK1/2 and p38 MAPK pathways
PMCID: Pubmed No.:26503828
DGAEE, a newly synthesized derivative of glycyrrhetinic acid, potently attenuates mouse septic shock via its main metabolite DGA in an IL-10-dependent manner
PMCID: Pubmed No.:26456500
A novel acetylcholinesterase inhibitor and calcium channel blocker SCR-1693 improves Aβ25–35-impaired mouse cognitive function
PMCID: Pubmed No.:26554390
Lipoxin A4 Attenuates Cell Invasion by Inhibiting ROS/ERK/MMP Pathway in Pancreatic Cancer
PMCID: Pubmed No.:26649143
Overexpression of miR-335 confers cell proliferation and tumour growth to colorectal carcinoma cells
PMCID: Pubmed No.:26708214
Dioscin suppresses human laryngeal cancer cells growth via induction of cell-cycle arrest and MAPK-mediated mitochondrial-derived apoptosis and inhibition of tumor invasion.
PMCID: Pubmed No.:26849940
MicroRNA-9 regulates cardiac fibrosis by targeting PDGFR-β in rats
PMCID: Pubmed No.:26896308
Dynamic monitoring of GPER-mediated estrogenic effects in breast cancer associated fibroblasts: an alternative role of estrogen in mammary carcinoma development
PMCID: Pubmed No.:27016131
TNF‐α downregulates CIDEC via MEK/ERK pathway in human adipocytes
PMCID: Pubmed No.:27062372
Fluid shear stress suppresses TNF-α-induced apoptosis in MC3T3-E1 cells: Involvement of ERK5-AKT-FoxO3a-Bim/FasL signaling pathways
PMCID: Pubmed No.:27060196
Fluid shear stress suppresses TNF-α-induced apoptosis in MC3T3-E1 cells: Involvement of ERK5-AKT-FoxO3a-Bim/FasL signaling pathways
PMCID: Pubmed No.:27060196
Aspirin suppresses TNF‑α‑induced MMP‑9 expression via NF‑κB and MAPK signaling pathways in RAW264.7 cells
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PKG II reverses HGF-triggered cellular activities by phosphorylating serine 985 of c-Met in gastric cancer cells
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The antimetastatic effect and underlying mechanisms of thioredoxin reductase inhibitor ethaselen
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Tropomodulin1 Expression Increases Upon Maturation in Dendritic Cells and Promotes Their Maturation and Immune Functions
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Hsa-let-7c controls the committed differentiation of IGF-1-treated mesenchymal stem cells derived from dental pulps by targeting IGF-1R via the MAPK pathways
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The anti-androgenic effects of cypermethrin mediated by non-classical testosterone pathway activation of mitogen-activated protein kinase cascade in mouse Sertoli cells
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Anti-neuroinflammatory Effect of Short-Chain Fatty Acid Acetate against Alzheimer’s Disease via Upregulating GPR41 and Inhibiting ERK/JNK/NF-κB
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Identification of extracellular vesicles-transported miRNAs in Erlotinib-resistant head and neck squamous cell carcinoma
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A novel biscoumarin derivative dephosphorylates ERK and alleviates apoptosis induced by mitochondrial oxidative damage in ischemic stroke mice
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A Biomimicking Polymeric Cryogel Scaffold for Repair of Critical-Sized Cranial Defect in a Rat Model
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Estrogen promotes the metastasis of non‑small cell lung cancer via estrogen receptor β by upregulation of Toll‑like receptor 4 and activation of the myd88/NF‑κB/MMP2 pathway
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Traditional Chinese Medicine CFF‐1 induced cell growth inhibition, autophagy, and apoptosis via inhibiting EGFR‐related pathways in prostate cancer
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Traditional Chinese Medicine CFF‐1 induced cell growth inhibition, autophagy, and apoptosis via inhibiting EGFR‐related pathways in prostate cancer
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Protective effect of bone marrow mesenchymal stem cell-derived exosomes against the reproductive toxicity of cyclophosphamide is associated with the p38MAPK/ERK and AKT signaling pathways
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Sub‐chronic administration of benzo[a]pyrene disrupts hippocampal long‐term potentiation via inhibiting CaMK II/PKC/PKA‐ERK‐CREB signaling in rats
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Let-7b regulates the adriamycin resistance of chronic myelogenous leukemia by targeting AURKB in K562/ADM cells
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Activation of RAW264.7 macrophage by Exopolysaccharide from Aphanothece halaphytica (EPSAH) and the underlying mechanisms
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4‐Sulfonyloxy/alkoxy benzoxazolone derivatives with high anti‐inflammatory activities: Synthesis, biological evaluation, and action mechanism via p38/ERK‐NF‐κB/iNOS pathway
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-Hederin Arrests Cell Cycle at G2/M Checkpoint and Promotes Mitochondrial Apoptosis by Blocking Nuclear Factor-B Signaling in Colon Cancer Cells
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Cross regulation of signaling pathways in gastrointestinal stromal tumor
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L-Theanine Down-Regulates the JAK/STAT3 Pathway to Attenuate the Proliferation and Migration of Vascular Smooth Muscle Cells Induced by Angiotensin II
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EGFR‑associated pathways involved in traditional Chinese medicine (TCM)‑1‑induced cell growth inhibition, autophagy and apoptosis in prostate cancer
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Zurampic Protects Pancreatic β-Cells from High Uric Acid Induced-Damage by Inhibiting URAT1 and Inactivating the ROS/AMPK/ERK Pathways
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Puerarin alleviates depressive-like behaviors in high-fat diet-induced diabetic mice via modulating hippocampal GLP-1R/BDNF/TrkB signaling
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Delivery of miR-3529-3p using MnO2-SiO2-APTES nanoparticles combined with phototherapy suppresses lung adenocarcinoma progression by targeting HIGD1A
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.