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14-3-3 gamma Recombinant Rabbit mAb BS45610
  • Western blot detection of 14-3-3 gamma/YWHAG in HL-60,U251 cell lysates using 14-3-3 gamma/YWHAG antibody(1:1000 diluted).
Product Name14-3-3 gamma Recombinant Rabbit mAb
Catalog No.BS45610
Swiss-ProtP61981
Host Rabbit
ReactivityHuman, Mouse, Rat
ApplicationsWB
Application_allWB: 1:1000
BiowMW28 kDa
Alternative NameEIEE56; PPP1R170; 14-3-3GAMMA
Purification&PurityAffinity Purification
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50 ul $258
100 ul $398
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Product Name :
14-3-3 gamma Recombinant Rabbit mAb
Background :
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]
Product :
Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human 14-3-3 gamma/YWHAG
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot detection of 14-3-3 gamma/YWHAG in HL-60,U251 cell lysates using 14-3-3 gamma/YWHAG antibody(1:1000 diluted).
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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