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HSF1 Recombinant Rabbit mAb BS46218
  • Western blot detection of HSF1 in Hela,Jurkat cell lysates using HSF1 antibody(1:500 diluted).
Product NameHSF1 Recombinant Rabbit mAb
Catalog No.BS46218
Swiss-ProtQ00613
Host Rabbit
ReactivityHuman
ApplicationsWB,IHC,ICC/IF,FC,IP
Application_allWB: 1:2000
IHC: 1:20
ICC/IF: 1:20-1:50
FC: 1:100
IP: 1:20
BiowMW80 kDa
Alternative NameHSTF1
Purification&PurityAffinity Purification
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50 ul $258
100 ul $398
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Product Name :
HSF1 Recombinant Rabbit mAb
Background :
The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Jul 2017]
Product :
Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human HSF1
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot detection of HSF1 in Hela,Jurkat cell lysates using HSF1 antibody(1:500 diluted).
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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