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HMGCL Recombinant Rabbit mAb BS47199
  • Western blot detection of HMGCL in Hela cell lysates using HMGCL antibody(1:1000 diluted).
Product NameHMGCL Recombinant Rabbit mAb
Catalog No.BS47199
Swiss-ProtP35914
Host Rabbit
ReactivityHuman
ApplicationsWB,IHC
Application_allWB: 1:1000
IHC: 1:100
BiowMW34 kDa
Alternative NameHL
Purification&PurityAffinity Purification
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50 ul $258
100 ul $398
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Product Name :
HMGCL Recombinant Rabbit mAb
Background :
The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Product :
Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthetic peptide of human HMGCL
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot detection of HMGCL in Hela cell lysates using HMGCL antibody(1:1000 diluted).
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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