Product Name :
Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) Background :
Monomethyl auristatin E (MMAE) is a synthetic derivative of dolastatin 10 and functions as a potent mitotic inhibitor by inhibiting tubulin polymerization. MMAE is widely used as a cytotoxic component of antibody-drug conjugates (ADCs) to treat several cancer types. Anti-MMAE antibody is a rabbit monoclonal antibody specially react with MMAE without MMAF, which is more sensitive than mouse antibody. The anti-MMAE antibody is a useful reagent in PK assay to determine conjugated antibodies. Product :
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. Storage&Stability :
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. Specificity :
Specifically recognizes MMAE and does not recognize MMAF. Immunogen :
MMAE-BSA Conjugate :
Unconjugated Modification :
Unmodified

• 
  Immobilized Disitamab Vedotin (RC48) at 0.2 μg/mL (100 μL/well) can bind Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) with a linear range of 0.06-2 ng/mL (QC tested).
• 
  Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) at 5 μg/mL, add Disitamab Vedotin (RC48) in the 100% Human Serum and then add Biotinylated Human Her2, His,Avitag, premium grade at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (QC tested).
• 
  Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) at 5 μg/mL, add Disitamab Vedotin (RC48) in the 100% Human Serum and then add Biotinylated Human Her2, His,Avitag, premium grade at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (QC tested).
• 
  Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) at 5 μg/mL, add Disitamab Vedotin (RC48) in the 100% Human Serum and then add Biotinylated Human Her2, His,Avitag, premium grade at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (QC tested).

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---