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PDHB Recombinant Rabbit mAb BS66208
  • Western blot analysis of PDHB expression in K562 (A), Jurkat (B), THP1 (C), mouse liver (D), mouse muscle (E), rat liver (F), rat muscle (G) whole cell lysates.
Product NamePDHB Recombinant Rabbit mAb
Catalog No.BS66208
Swiss-ProtP11177
Host Rabbit
ReactivityHuman, Mouse, Rat
ApplicationsWB
Application_allWB,1:500 - 1:1000
BiowMW~ 35 kDa
Alternative NamePHE1B; Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; PDHE1-B
Purification&PurityThe antibody was affinity-purified from cell culture supernatant by protein A+G and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $168
100ul $298
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Product Name :
PDHB Recombinant Rabbit mAb
Background :
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
PDHB mAb detects endogenous levels of PDHB protein.
Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within human PDHB protein.
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of PDHB expression in K562 (A), Jurkat (B), THP1 (C), mouse liver (D), mouse muscle (E), rat liver (F), rat muscle (G) whole cell lysates.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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