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GLS polyclonal antibody BS66209
  • Western blot analysis of GLS expression in SW620 (A), mouse lung (B) whole cell lysates.
  • mmunohistochemical analysis of GLS staining in human brain formalin fixed paraffin embedded tissue section.
  • mmunohistochemical analysis of GLS staining in human brain formalin fixed paraffin embedded tissue section.
Product NameGLS polyclonal antibody
Catalog No.BS66209
Swiss-ProtO94925
Host Rabbit
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF/ICC
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200 IF/ICC: 1:50~1:200
BiowMW~ 73 kDa
Alternative NameGLS1; KIAA0838; Glutaminase kidney isoform mitochondrial; GLS; K-glutaminase; L-glutamine amidohydrolase
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $158
100ul $275
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Product Name :
GLS polyclonal antibody
Background :
Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity. Isoform 1 and isoform 3 are activated by phosphate. Inhibited by BPTES. BPTES binds between subunits and favors dissociation of the tetramer into dimers.
Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
GLS pAb detects endogenous levels of GLS protein.
Immunogen :
Recombinant full length protein of human GLS
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of GLS expression in SW620 (A), mouse lung (B) whole cell lysates.
  • mmunohistochemical analysis of GLS staining in human brain formalin fixed paraffin embedded tissue section.
  • mmunohistochemical analysis of GLS staining in human brain formalin fixed paraffin embedded tissue section.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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