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Recombinant Human BAFF Receptor (rHuBAFF-R) PR1003
RecombinantRecombinant Human BAFF Receptor (rHuBAFF-R)
Catalog No.PR1003
SourceEscherichia coli.
Molecular WeightApproximately 7.7 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.
Purity>95% by reduced SDS-PAGE analyses.
Biological ActivityFully biologically active when compared to standard. Determined by its ability to block BAFF induced mouse splenocyte survival. The expected ED50 for this effect is 1.0-5.0 μg/ml in the presence of 1.0μg/ml of human soluble BAFF, corresponding to a Specific Activity of >200 IU/mg.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationLyophilized from a 0.2mm filtered concentrated solution in 20mM PB, pH 8.0, 500mM NaCl.
EndotoxinLess than 1EU/mg of rHuBAFF-R as determined by LAL method.
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50µg $298
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Recombinant :
Recombinant Human BAFF Receptor (rHuBAFF-R)
Source :
Escherichia coli.
Molecular Weight :
Approximately 7.7 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.
Purity :
>95% by reduced SDS-PAGE analyses.
Biological Activity :
Fully biologically active when compared to standard. Determined by its ability to block BAFF induced mouse splenocyte survival. The expected ED50 for this effect is 1.0-5.0 μg/ml in the presence of 1.0μg/ml of human soluble BAFF, corresponding to a Specific Activity of >200 IU/mg.
Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation :
Lyophilized from a 0.2mm filtered concentrated solution in 20mM PB, pH 8.0, 500mM NaCl.
AA Sequence :
MRRGPRSLRG RDAPAPTPCV PAECFDLLVR HCVACGLLRT PRPKPAGASS PAPRTALQPQ ESVGAGAGEA ALPLPG
Endotoxin :
Less than 1EU/mg of rHuBAFF-R as determined by LAL method.
Reconstitution :
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage :
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China
Description :
BAFF Receptor (BAFF-R), a member of the TNFR superfamily, is highly expressed in spleen, lymph node, and resting B cells and to some extent in activated B cells, resting CD4+ cells and peripheral blood leukocytes. BAFF-R is a type III transmembrane protein that binds with high specificity to BAFF (TNFSF13B). BAFF-R/BAFF signaling plays a critical role in B cell survival and maturation.
Blocking peptide available as PR1003P
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