Recombinant Murine Interleukin-3
(rMuIL-3 )
Datasheet
COA
MSDS
SHIP
Recombinant :
Recombinant Murine Interleukin-3 (rMuIL-3 ) Source :
Escherichia coli. Molecular Weight :
Approximately 15.1 kDa globular protein containing 135 amino acid residues. Purity :
>98% by SDS-PAGE and HPLC analyses. Biological Activity :
The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is < 0.05 ng/ml, corresponding to a specific activity of > 2 x 107 units/mg. Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4. AA Sequence :
MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC Endotoxin :
Less than 1EU/mg of rmIL-3 as determined by LAL method. Reconstitution :
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions. Storage :
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China Description :
IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine.
Recombinant Murine Interleukin-3 (rMuIL-3 ) Source :
Escherichia coli. Molecular Weight :
Approximately 15.1 kDa globular protein containing 135 amino acid residues. Purity :
>98% by SDS-PAGE and HPLC analyses. Biological Activity :
The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is < 0.05 ng/ml, corresponding to a specific activity of > 2 x 107 units/mg. Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4. AA Sequence :
MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC Endotoxin :
Less than 1EU/mg of rmIL-3 as determined by LAL method. Reconstitution :
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions. Storage :
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China Description :
IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine.
Blocking peptide available as PR2023P