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Recombinant Murine Interleukin-33 (rMuIL-33 ) PR2024
RecombinantRecombinant Murine Interleukin-33 (rMuIL-33 )
Catalog No.PR2024
SourceEscherichia coli.
Molecular WeightApproximately 17.5 kDa protein containing 158 amino acid residues
Purity>98% by SDS-PAGE and HPLC analyses.
Biological ActivityThe ED50 was determined by the dose-dependent stimulation of the proliferation of murine D10S cells is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 106units/mg.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationLyophilized from a 0.2mm filtered solution in PBS, EDTA and DTT.
EndotoxinLess than 1EU/mg of rmIL-33 as determined by LAL method.
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10µg $298
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Recombinant :
Recombinant Murine Interleukin-33 (rMuIL-33 )
Source :
Escherichia coli.
Molecular Weight :
Approximately 17.5 kDa protein containing 158 amino acid residues
Purity :
>98% by SDS-PAGE and HPLC analyses.
Biological Activity :
The ED50 was determined by the dose-dependent stimulation of the proliferation of murine D10S cells is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 106units/mg.
Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation :
Lyophilized from a 0.2mm filtered solution in PBS, EDTA and DTT.
AA Sequence :
SIQGTSLLTQ SPASLSTYND QSVSFVLENG CYVINVDDSG KDQEQDQVLL RYYESPCPAS QSGDGVDGKK LMVNMSPIKD TDIWLHANDK DYSVELQRGD VSPPEQAFFV LHKKSSDFVS FECKNLPGTY IGVKDNQLAL VEEKDESCNN IMFKLSKI
Endotoxin :
Less than 1EU/mg of rmIL-33 as determined by LAL method.
Reconstitution :
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage :
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China
Description :
IL-33 is a proinflammatory protein that shares structural and functional characteristics with the IL-1 cytokine family. It binds and signals through the IL-1RL1/ST2 receptor activating NF-kappaB and MAP kinases. IL-33 induces production of TH2 cell related cytokines, including IL-4, IL-5 and IL-13, and exerts multiple inflammation related bioactivities.
Blocking peptide available as PR2024P
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