Recombinant FGF-8f, Human 
Datasheet
COA
MSDS
SHIP
Recombinant :
Recombinant FGF-8f, Human Source :
Escherichia coli. Molecular Weight :
25.5 kDa, observed by reducing SDS-PAGE. Purity :
> 95% by SDS-PAGE analysis. Biological Activity :
ED50 < 50ng/mL, measured by a cell proliferation assayusing 3T3 cellsin the presence of 10 μg/ml of heparin, corresponding to a specific activity of > 2× 10ˆ4 units/mg. Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized after extensive dialysis against PBS. AA Sequence :
MQEGPGRGP ALGRELASLF RAGREPQGVS QQVTVQSSPN FTQHVREQSL VTDQLSRRLI RTYQLYSRTS GKHVQVLANK RINAMAEDGD PFAKLIVETD TFGSRVRVRG AETGLYICMN KKGKLIAKSN GKGKDCVFTE IVLENNYTAL QNAKYEGWYM AFTRKGRPRK GSKTRQHQRE VHFMKRLPRG HHTTEQSLRF EFLNYPPFTR SLRGSQRTWA PEPR Endotoxin :
< 0.2 EU/μg, determined by LAL method. Reconstitution :
Reconstituted in ddH2O at 100 μg/mL. Storage :
Lyophilized recombinant human Fibroblast Growth Factor 8f(rhFGF-8f) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-8f remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description :
Fibroblast Growth Factor 8f (FGF-8f) is a cytokine belonging to the heparin-binding FGF family, which has at least 23 members. FGF-8 has 8 different isoforms, named FGF-8a through FGF-8h. Different FGF-8 isoforms have different receptor affinities, and thus participate in different signaling cascade pathways. FGF-8 has widespread expression during embryonic development, promoting gastrulation, somitogenesis, morphogenesis, and limb formation. FGF-8 also has oncogenic potential. While in normal cells FGF-8 is expressed at very low levels, in breast, prostate and ovarian cancer FGF-8 is highly expressed.FGF-8 promotes tumor angiogenesis by increasing neovascularization, and inducing osteoblastic differentiation. Recombinant human Fibroblast Growth Factor 8f (rhFGF-8f) produced in E.coli is a single non-glycosylated polypeptide chain containing 223 amino acids. A fully biologically active molecule, rhFGF-8f has a molecular mass of 25.5 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Recombinant FGF-8f, Human Source :
Escherichia coli. Molecular Weight :
25.5 kDa, observed by reducing SDS-PAGE. Purity :
> 95% by SDS-PAGE analysis. Biological Activity :
ED50 < 50ng/mL, measured by a cell proliferation assayusing 3T3 cellsin the presence of 10 μg/ml of heparin, corresponding to a specific activity of > 2× 10ˆ4 units/mg. Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized after extensive dialysis against PBS. AA Sequence :
MQEGPGRGP ALGRELASLF RAGREPQGVS QQVTVQSSPN FTQHVREQSL VTDQLSRRLI RTYQLYSRTS GKHVQVLANK RINAMAEDGD PFAKLIVETD TFGSRVRVRG AETGLYICMN KKGKLIAKSN GKGKDCVFTE IVLENNYTAL QNAKYEGWYM AFTRKGRPRK GSKTRQHQRE VHFMKRLPRG HHTTEQSLRF EFLNYPPFTR SLRGSQRTWA PEPR Endotoxin :
< 0.2 EU/μg, determined by LAL method. Reconstitution :
Reconstituted in ddH2O at 100 μg/mL. Storage :
Lyophilized recombinant human Fibroblast Growth Factor 8f(rhFGF-8f) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-8f remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description :
Fibroblast Growth Factor 8f (FGF-8f) is a cytokine belonging to the heparin-binding FGF family, which has at least 23 members. FGF-8 has 8 different isoforms, named FGF-8a through FGF-8h. Different FGF-8 isoforms have different receptor affinities, and thus participate in different signaling cascade pathways. FGF-8 has widespread expression during embryonic development, promoting gastrulation, somitogenesis, morphogenesis, and limb formation. FGF-8 also has oncogenic potential. While in normal cells FGF-8 is expressed at very low levels, in breast, prostate and ovarian cancer FGF-8 is highly expressed.FGF-8 promotes tumor angiogenesis by increasing neovascularization, and inducing osteoblastic differentiation. Recombinant human Fibroblast Growth Factor 8f (rhFGF-8f) produced in E.coli is a single non-glycosylated polypeptide chain containing 223 amino acids. A fully biologically active molecule, rhFGF-8f has a molecular mass of 25.5 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Blocking peptide available as BK0048-50μgP