Recombinant GM-CSF, Human 
Datasheet
COA
MSDS
SHIP
Recombinant :
Recombinant GM-CSF, Human Source :
Escherichia coli. Molecular Weight :
14 kDa, observed by SDS-PAGE. Purity :
>95 % as analyzed by SDS-PAGE. Biological Activity :
ED50 < 0.5 ng/ml, measured by proliferation assay of TF-1 cells, corresponding to a specific activity of >2.0 x 10ˆ6units/mg Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized after extensive dialysis against 10 mM PB, pH7.0. AA Sequence :
MAPARSPSP STQPWEHVNA IQEARRLLNL SRDTAAEMNE TVEVISEMFD LQEPTCLQTR LELYKQGLRG SLTKLKGPLT MMASHYKQHC PPTPETSCAT QIITFESFKE NLKDFLLVIP FDCWEPVQE Endotoxin :
<1 EU/μg, determined by LAL method. Reconstitution :
Reconstituted in ddH2O at 100μg/ml. Storage :
Lyophilized recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhGM-CSF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description :
Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF), a hematopoietic growth factor, is mainly involved in granulopoiesis and monocytopoiesis. It is produced by T-cells and macrophages in response to antigens, and by endothelial cells and fibroblasts following induction of variouscytokines [1]. A monomeric protein of 127 amino acidswith six glycosylation sites and two intra disulfide bonds [2,3], glycosylated and non-glycosylated hGM-CSFs show similar biological activities [4].Other than its connection to the growth and development of granulocytes and macrophages, it is also indispensable for the proliferation of erythroid and megakaryocytic cells [5].Recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) produced in E.coli is a non-glycosylated polypeptide. A fully biologically active molecule, rhGM-CSF has a molecular mass of 14 kDa analyzed by SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Recombinant GM-CSF, Human Source :
Escherichia coli. Molecular Weight :
14 kDa, observed by SDS-PAGE. Purity :
>95 % as analyzed by SDS-PAGE. Biological Activity :
ED50 < 0.5 ng/ml, measured by proliferation assay of TF-1 cells, corresponding to a specific activity of >2.0 x 10ˆ6units/mg Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized after extensive dialysis against 10 mM PB, pH7.0. AA Sequence :
MAPARSPSP STQPWEHVNA IQEARRLLNL SRDTAAEMNE TVEVISEMFD LQEPTCLQTR LELYKQGLRG SLTKLKGPLT MMASHYKQHC PPTPETSCAT QIITFESFKE NLKDFLLVIP FDCWEPVQE Endotoxin :
<1 EU/μg, determined by LAL method. Reconstitution :
Reconstituted in ddH2O at 100μg/ml. Storage :
Lyophilized recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhGM-CSF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description :
Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF), a hematopoietic growth factor, is mainly involved in granulopoiesis and monocytopoiesis. It is produced by T-cells and macrophages in response to antigens, and by endothelial cells and fibroblasts following induction of variouscytokines [1]. A monomeric protein of 127 amino acidswith six glycosylation sites and two intra disulfide bonds [2,3], glycosylated and non-glycosylated hGM-CSFs show similar biological activities [4].Other than its connection to the growth and development of granulocytes and macrophages, it is also indispensable for the proliferation of erythroid and megakaryocytic cells [5].Recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) produced in E.coli is a non-glycosylated polypeptide. A fully biologically active molecule, rhGM-CSF has a molecular mass of 14 kDa analyzed by SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Blocking peptide available as BK0066-50μgP