Recombinant IL-1β, Human 
Datasheet
COA
MSDS
SHIP
Recombinant :
Recombinant IL-1β, Human Source :
Escherichia coli. Molecular Weight :
17.3 kDa, observed by reducing SDS-PAGE. Purity :
> 95% by SDS-PAGE and HPLC analyses. Biological Activity :
ED50< 1.0 pg/ml, measured by the dose-dependent stimulation of mouse D10S cells, corresponding to a specific activity of 1.0× 10ˆ9 IU/mg. Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized after extensive dialysis against PBS. AA Sequence :
APV RSLNCTLRDS QQKSLVMSGP YELKALHLQG QDMEQQVVFS MSFVQGEESN DKIPVALGLK EKNLYLSCVL KDDKPTLQLE SVDPKNYPKK KMEKRFVFNK IEINNKLEFE SAQFPNWYIS TSQAENMPVF LGGTKGGQDI TDFTMQFVSS Endotoxin :
<0.2 EU/μg, determined by LAL method. Reconstitution :
Reconstituted in ddH2O or PBS at 100 μg/ml. Storage :
Lyophilized recombinant human Interleukin-1 beta (rhIL-1β) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIL-1β should be stable up to 2 weeks at 4°C or up to 3 months at -20°C. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description :
Interleukin-1 beta (rhIL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.Recombinant human Interleukin-1 beta (rhIL-1β) produced in E.coli is a single non-glycosylated polypeptide chain containing 153 amino acids. A fully biologically active molecule, rhIL-1β has a molecular mass of 17.3kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Recombinant IL-1β, Human Source :
Escherichia coli. Molecular Weight :
17.3 kDa, observed by reducing SDS-PAGE. Purity :
> 95% by SDS-PAGE and HPLC analyses. Biological Activity :
ED50< 1.0 pg/ml, measured by the dose-dependent stimulation of mouse D10S cells, corresponding to a specific activity of 1.0× 10ˆ9 IU/mg. Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized after extensive dialysis against PBS. AA Sequence :
APV RSLNCTLRDS QQKSLVMSGP YELKALHLQG QDMEQQVVFS MSFVQGEESN DKIPVALGLK EKNLYLSCVL KDDKPTLQLE SVDPKNYPKK KMEKRFVFNK IEINNKLEFE SAQFPNWYIS TSQAENMPVF LGGTKGGQDI TDFTMQFVSS Endotoxin :
<0.2 EU/μg, determined by LAL method. Reconstitution :
Reconstituted in ddH2O or PBS at 100 μg/ml. Storage :
Lyophilized recombinant human Interleukin-1 beta (rhIL-1β) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIL-1β should be stable up to 2 weeks at 4°C or up to 3 months at -20°C. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description :
Interleukin-1 beta (rhIL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.Recombinant human Interleukin-1 beta (rhIL-1β) produced in E.coli is a single non-glycosylated polypeptide chain containing 153 amino acids. A fully biologically active molecule, rhIL-1β has a molecular mass of 17.3kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Blocking peptide available as BK0094-50μgP