Recombinant IL-33, Human 
Datasheet
COA
MSDS
SHIP
Recombinant :
Recombinant IL-33, Human Source :
Escherichia coli. Molecular Weight :
18.0 kDa, observed by reducing SDS-PAGE. Purity :
> 95% by SDS-PAGEanalysis. Biological Activity :
ED50 <0.5ng/mL, measured by a cell proliferation assay using D10S cells, corresponding to a specific activity of > 2× 10ˆ6 units/mg. Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized after extensive dialysis against PBS. AA Sequence :
SIT GISPITEYLA SLSTYNDQSI TFALEDESYE IYVEDLKKDE KKDKVLLSYY ESQHPSNESG DGVDGKMLMVTLSPTKDFWL HANNKEHSVE LHKCEKPLPD QAFFVLHNMH SNCVSFECKT DPGVFIGVKD NHLALIKVDS SENLCTENIL FKLSET Endotoxin :
< 0.2 EU/μg, determined by LAL method. Reconstitution :
Reconstituted in ddH2O at 100 μg/mL. Storage :
Lyophilized recombinant human Interleukin-33 (rhIL-33) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIL-33 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description :
Interleukin-33 (IL-33) is a proinflammatory cytokine that belongs to the IL-1 family. IL-33 is expressed in a variety of cells, including epithelial and endothelial cells, smooth muscle cells, macrophages and fibroblasts. The primary receptors for IL-33 are ST2 and IL-1 receptor accessory protein (IL-1RAcP), both of which belong to the IL-1 receptor family. IL-33 is localized to the nucleus of resting cells where it binds to chromatin in the H2A-H2B histone complex as a transcriptional suppressor. IL-33 is secreted by cells during injury which induces a T-helper 2 type inflammatory response. Evidence suggests IL-33 plays a role in autoimmune disease. IL-33’s interaction with ST2 can drive allergic pathology and IL-33 has been reported to play a role in the development of rheumatoid arthritis and systemic lupus erythematosus.Recombinant humanInterleukin-33 (rhIL-33) produced in E.coli is a single non-glycosylated polypeptide chain containing 159 amino acids. A fully biologically active molecule, rhIL-33 has a molecular mass of 18.0 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Recombinant IL-33, Human Source :
Escherichia coli. Molecular Weight :
18.0 kDa, observed by reducing SDS-PAGE. Purity :
> 95% by SDS-PAGEanalysis. Biological Activity :
ED50 <0.5ng/mL, measured by a cell proliferation assay using D10S cells, corresponding to a specific activity of > 2× 10ˆ6 units/mg. Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized after extensive dialysis against PBS. AA Sequence :
SIT GISPITEYLA SLSTYNDQSI TFALEDESYE IYVEDLKKDE KKDKVLLSYY ESQHPSNESG DGVDGKMLMVTLSPTKDFWL HANNKEHSVE LHKCEKPLPD QAFFVLHNMH SNCVSFECKT DPGVFIGVKD NHLALIKVDS SENLCTENIL FKLSET Endotoxin :
< 0.2 EU/μg, determined by LAL method. Reconstitution :
Reconstituted in ddH2O at 100 μg/mL. Storage :
Lyophilized recombinant human Interleukin-33 (rhIL-33) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIL-33 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description :
Interleukin-33 (IL-33) is a proinflammatory cytokine that belongs to the IL-1 family. IL-33 is expressed in a variety of cells, including epithelial and endothelial cells, smooth muscle cells, macrophages and fibroblasts. The primary receptors for IL-33 are ST2 and IL-1 receptor accessory protein (IL-1RAcP), both of which belong to the IL-1 receptor family. IL-33 is localized to the nucleus of resting cells where it binds to chromatin in the H2A-H2B histone complex as a transcriptional suppressor. IL-33 is secreted by cells during injury which induces a T-helper 2 type inflammatory response. Evidence suggests IL-33 plays a role in autoimmune disease. IL-33’s interaction with ST2 can drive allergic pathology and IL-33 has been reported to play a role in the development of rheumatoid arthritis and systemic lupus erythematosus.Recombinant humanInterleukin-33 (rhIL-33) produced in E.coli is a single non-glycosylated polypeptide chain containing 159 amino acids. A fully biologically active molecule, rhIL-33 has a molecular mass of 18.0 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Blocking peptide available as BK0104-10μgP