Recombinant MCP-2/CCL8, Human 
Datasheet
COA
MSDS
SHIP
Recombinant :
Recombinant MCP-2/CCL8, Human Source :
Escherichia coli. Molecular Weight :
8.9 kDa, observed by reducing SDS-PAGE. Purity :
> 95% by SDS-PAGE analysis. Biological Activity :
ED50< 0.5 µg/mL, measured by the FLIPR assay using CHO cells transfected with human CCR5, the receptor of human CCL8, corresponding to a specific activity of > 2 × 10ˆ3 units/mg. Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized after extensive dialysis against PBS. AA Sequence :
QPD SVSIPITCCF NVINRKIPIQ RLESYTRITN IQCPKEAVIF KTKRGKEVCA DPKERWVRDS MKHLDQIFQN LKP Endotoxin :
< 0.2 EU/μg, determined by LAL method. Reconstitution :
Reconstituted in ddH2O at 100 μg/mL. Storage :
Lyophilized recombinant human MCP-2/CCL8(rhMCP-2) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhMCP-2 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description :
MCP-2 is a member of the chemokines, a group of 70-80 residue proteins sharing substantial sequence similarity. Within the chemokines, MCP-2 belongs to the CC subfamily, and is a member of the Monocyte Chemoattractant Proteins (MCPs), which includes MCP-1, MCP-2, MCP-3, MCP-4, and MCP-5. MCP-2 shares 60% homology with MCP-1, and both proteins can undergo reversible dimerization. The main receptors of MCP-2 are G-protein coupled receptors CCR1 and CCR5. MCP-2 is a potential target in HIV-1 infected human glial cells as it may play a role in the modulation of viral spread in the brain. Recently, researchers found that mouse MCP-2 is expressed in the skin as a novel agonist of CCR8 and plays a role in eosinophilic inflammation.Recombinant human MCP-2/CCL8(rhMCP-2) produced in E.coli is a single non-glycosylated polypeptide chain containing 76 amino acids. A fully biologically active molecule, rhMCP-2 has a molecular mass of 8.9kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Recombinant MCP-2/CCL8, Human Source :
Escherichia coli. Molecular Weight :
8.9 kDa, observed by reducing SDS-PAGE. Purity :
> 95% by SDS-PAGE analysis. Biological Activity :
ED50< 0.5 µg/mL, measured by the FLIPR assay using CHO cells transfected with human CCR5, the receptor of human CCL8, corresponding to a specific activity of > 2 × 10ˆ3 units/mg. Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized after extensive dialysis against PBS. AA Sequence :
QPD SVSIPITCCF NVINRKIPIQ RLESYTRITN IQCPKEAVIF KTKRGKEVCA DPKERWVRDS MKHLDQIFQN LKP Endotoxin :
< 0.2 EU/μg, determined by LAL method. Reconstitution :
Reconstituted in ddH2O at 100 μg/mL. Storage :
Lyophilized recombinant human MCP-2/CCL8(rhMCP-2) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhMCP-2 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description :
MCP-2 is a member of the chemokines, a group of 70-80 residue proteins sharing substantial sequence similarity. Within the chemokines, MCP-2 belongs to the CC subfamily, and is a member of the Monocyte Chemoattractant Proteins (MCPs), which includes MCP-1, MCP-2, MCP-3, MCP-4, and MCP-5. MCP-2 shares 60% homology with MCP-1, and both proteins can undergo reversible dimerization. The main receptors of MCP-2 are G-protein coupled receptors CCR1 and CCR5. MCP-2 is a potential target in HIV-1 infected human glial cells as it may play a role in the modulation of viral spread in the brain. Recently, researchers found that mouse MCP-2 is expressed in the skin as a novel agonist of CCR8 and plays a role in eosinophilic inflammation.Recombinant human MCP-2/CCL8(rhMCP-2) produced in E.coli is a single non-glycosylated polypeptide chain containing 76 amino acids. A fully biologically active molecule, rhMCP-2 has a molecular mass of 8.9kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Blocking peptide available as BK0126-25μgP