Recombinant MIP-1α/CCL3, Human 
Datasheet
COA
MSDS
SHIP
Recombinant :
Recombinant MIP-1α/CCL3, Human Source :
Escherichia coli. Molecular Weight :
7.8 kDa, observed by reducing SDS-PAGE. Purity :
> 95% as analyzed by SDS-PAGE and HPLC. Biological Activity :
ED50 < 80 ng/mL, measured by the FLIPR assay using CHO cells transfected with human CCR5, the receptor of human CCL3, corresponding to a specific activity of > 1.25×10ˆ4 units/mg. Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized after extensive dialysis against PBS. AA Sequence :
ASL AADTPTACCF SYTSRQIPQN FIADYFETSS QCSKPGVIFL TKRSRQVCAD PSEEWVQKYV SDLELSA Endotoxin :
< 0.2 EU/μg, determined by LAL method. Reconstitution :
Reconstituted in ddH2O or PBS at 100 μg/ml. Storage :
Lyophilized recombinant human MIP-1 alpha/CCL3 (rhMIP-1 alpha) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhMIP-1 alpha remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description :
MIP-1 alpha/CCL3, also known as LD78 alpha, is an inflammatory chemokine. MIP-1α belongs to the CCL chemokine family, and shares 68% homology with MIP-1β. The mature form of MIP-1α contains 69 amino acids, exists as dimers in solution, and tends to undergo reversible aggregation. The receptors of MIP-1αin vivo are mainly the G-protein coupled receptors CCR1 and CCR5. Upon stimulation by endogenous and exogenous agents such as Interleukin-1β, Interferon-γ, and lipoteichoic acid from Gram-positive bacteria, monocytes are able to secrete significant amounts of MIP-1α. MIP-1α augments the adhesions of T lymphocytes, monocytes, and neutrophils to vascular cell adhesion molecule 1. In addition, in wounds, MIP-1α chemo-attracts macrophages in order to accelerate the tissue repair process.Recombinant human MIP-1 alpha/CCL3 (rhMIP-1 alpha) produced in E.coli is a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhMIP-1 alpha has a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Recombinant MIP-1α/CCL3, Human Source :
Escherichia coli. Molecular Weight :
7.8 kDa, observed by reducing SDS-PAGE. Purity :
> 95% as analyzed by SDS-PAGE and HPLC. Biological Activity :
ED50 < 80 ng/mL, measured by the FLIPR assay using CHO cells transfected with human CCR5, the receptor of human CCL3, corresponding to a specific activity of > 1.25×10ˆ4 units/mg. Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized after extensive dialysis against PBS. AA Sequence :
ASL AADTPTACCF SYTSRQIPQN FIADYFETSS QCSKPGVIFL TKRSRQVCAD PSEEWVQKYV SDLELSA Endotoxin :
< 0.2 EU/μg, determined by LAL method. Reconstitution :
Reconstituted in ddH2O or PBS at 100 μg/ml. Storage :
Lyophilized recombinant human MIP-1 alpha/CCL3 (rhMIP-1 alpha) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhMIP-1 alpha remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description :
MIP-1 alpha/CCL3, also known as LD78 alpha, is an inflammatory chemokine. MIP-1α belongs to the CCL chemokine family, and shares 68% homology with MIP-1β. The mature form of MIP-1α contains 69 amino acids, exists as dimers in solution, and tends to undergo reversible aggregation. The receptors of MIP-1αin vivo are mainly the G-protein coupled receptors CCR1 and CCR5. Upon stimulation by endogenous and exogenous agents such as Interleukin-1β, Interferon-γ, and lipoteichoic acid from Gram-positive bacteria, monocytes are able to secrete significant amounts of MIP-1α. MIP-1α augments the adhesions of T lymphocytes, monocytes, and neutrophils to vascular cell adhesion molecule 1. In addition, in wounds, MIP-1α chemo-attracts macrophages in order to accelerate the tissue repair process.Recombinant human MIP-1 alpha/CCL3 (rhMIP-1 alpha) produced in E.coli is a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhMIP-1 alpha has a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Blocking peptide available as BK0136-50μgP