Recombinant MIP-5/CCL15 (92aa), Human 
Datasheet
COA
MSDS
SHIP
Recombinant :
Recombinant MIP-5/CCL15 (92aa), Human Source :
Escherichia coli. Molecular Weight :
10.2 kDa, observed by reducing SDS-PAGE. Purity :
> 95% by SDS-PAGE analysis. Biological Activity :
ED50 < 2 µg/mL, measured by the FLIPR assay using CHO cells transfected with human CCR1, the receptor of human CCL15, corresponding to a specific activity of > 500 units/mg. Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized after extensive dialysis against PBS. AA Sequence :
QFT NDAETELMMS KLPLENPVVL NSFHFAADCC TSYISQSIPC SLMKSYFETS SECSKPGVIF LTKKGRQVCA KPSGPGVQDC MKKLKPYSI Endotoxin :
< 0.2 EU/μg, determined by LAL method. Reconstitution :
Reconstituted in ddH2O at 100 μg/mL. Storage :
Lyophilized recombinant human MIP-5/CCL15 (rhMIP-5/CCL15) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhMIP-5/CCL15 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description :
Macrophage Inflammatory Protein-5 (MIP-5/CCL15) is a chemokine originally identified in the human hemofiltrate, thus it is also named Hemofiltrate CC Chemokine-2 (HCC-2). MIP-5 belongs to the CCL chemokine family, and its receptors are G-protein coupled receptors CCR1 and CCR3, with CCR1 being the major one. MIP-5 is mainly expressed in heart and skeletal muscle, and CCR1 is expressed on Th1 and Th2 cells in human cord blood lymphocytes. In vivo, MIP-5 promotes the accumulation of immature myeloid cells and the expansion of metastatic foci in the lever. MIP-5 contributes to severe asthma, sarcoidosis, and atherosclerosis;however, MIP-5 can also inhibit stem cell proliferation, implicating its therapeutic potential as an alternative to high dose chemotherapy.Recombinant human MIP-5/CCL15 (rhMIP-5/CCL15) produced in E.coli is a single non-glycosylated polypeptide chain containing 92 amino acids. A fully biologically active molecule, rhMIP-5/CCL15 has a molecular mass of 10.2 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Recombinant MIP-5/CCL15 (92aa), Human Source :
Escherichia coli. Molecular Weight :
10.2 kDa, observed by reducing SDS-PAGE. Purity :
> 95% by SDS-PAGE analysis. Biological Activity :
ED50 < 2 µg/mL, measured by the FLIPR assay using CHO cells transfected with human CCR1, the receptor of human CCL15, corresponding to a specific activity of > 500 units/mg. Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized after extensive dialysis against PBS. AA Sequence :
QFT NDAETELMMS KLPLENPVVL NSFHFAADCC TSYISQSIPC SLMKSYFETS SECSKPGVIF LTKKGRQVCA KPSGPGVQDC MKKLKPYSI Endotoxin :
< 0.2 EU/μg, determined by LAL method. Reconstitution :
Reconstituted in ddH2O at 100 μg/mL. Storage :
Lyophilized recombinant human MIP-5/CCL15 (rhMIP-5/CCL15) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhMIP-5/CCL15 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description :
Macrophage Inflammatory Protein-5 (MIP-5/CCL15) is a chemokine originally identified in the human hemofiltrate, thus it is also named Hemofiltrate CC Chemokine-2 (HCC-2). MIP-5 belongs to the CCL chemokine family, and its receptors are G-protein coupled receptors CCR1 and CCR3, with CCR1 being the major one. MIP-5 is mainly expressed in heart and skeletal muscle, and CCR1 is expressed on Th1 and Th2 cells in human cord blood lymphocytes. In vivo, MIP-5 promotes the accumulation of immature myeloid cells and the expansion of metastatic foci in the lever. MIP-5 contributes to severe asthma, sarcoidosis, and atherosclerosis;however, MIP-5 can also inhibit stem cell proliferation, implicating its therapeutic potential as an alternative to high dose chemotherapy.Recombinant human MIP-5/CCL15 (rhMIP-5/CCL15) produced in E.coli is a single non-glycosylated polypeptide chain containing 92 amino acids. A fully biologically active molecule, rhMIP-5/CCL15 has a molecular mass of 10.2 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Blocking peptide available as BK0140-25μgP