Recombinant OSM (227aa), Human 
Datasheet
COA
MSDS
SHIP
Recombinant :
Recombinant OSM (227aa), Human Source :
Escherichia coli. Molecular Weight :
25.9 kDa, observed by reducing SDS-PAGE. Purity :
> 95% by SDS-PAGE analysis. Biological Activity :
ED50 < 10 ng/mL, measured by a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 1× 10ˆ5 units/mg. Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized after extensive dialysis against PBS. AA Sequence :
MAAIGSCSK EYRVLLGQLQ KQTDLMQDTS RLLDPYIRIQ GLDVPKLREH CRERPGAFPS EETLRGLGRR GFLQTLNATL GCVLHRLADL EQRLPKAQDL ERSGLNIEDL EKLQMARPNI LGLRNNIYCM AQLLDNSDTA EPTKAGRGAS QPPTPTPASD AFQRKLEGCR FLHGYHRFMH SVGRVFSKWG ESPNRSRRHS PHQALRKGVR RTRPSRKGKR LMTRGQLPR Endotoxin :
< 0.2 EU/μg, determined by LAL method. Reconstitution :
Reconstituted in ddH2O at 100 μg/mL. Storage :
Lyophilized recombinant human Oncostatin M (rhOSM) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhOSM should be stable up to 2 weeks at 4°C or up to 3 months at -20°C. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description :
Oncostatin M (OSM) is a multifunctional cytokine, and belongs to Interleukin-6 (IL-6) subfamily, including IL-11, leukemia inhibitory factor (LIF), ciliary neurotropic factor, cardiotrophin-1, and novel neurotropin-1. In vivo, OSM is secreted from activated T cells, monocytes, neutrophils, and endothelial cells. OSM is related to LIF, and share a receptor with LIF in human. Human OSM can bind to gp130 and recruit OSM Receptor β or LIF Receptor β to form a ternary complex. OSM stimulates the growth of different types of cells, including megakaryocytes, fibroblasts, vascular endothelial cells, and T cells. On the other hand, OSM inhibits the proliferation of several cancer cell lines, such as solid tissue tumor cells, lung cancer cells, melanoma cells, and breast cancer cells.Recombinant human Oncostatin M (rhOSM) produced in E.coli is a single non-glycosylated polypeptide chain containing 228 amino acids. A fully biologically active molecule, rhOSM has a molecular mass of 25.9 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Recombinant OSM (227aa), Human Source :
Escherichia coli. Molecular Weight :
25.9 kDa, observed by reducing SDS-PAGE. Purity :
> 95% by SDS-PAGE analysis. Biological Activity :
ED50 < 10 ng/mL, measured by a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 1× 10ˆ5 units/mg. Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized after extensive dialysis against PBS. AA Sequence :
MAAIGSCSK EYRVLLGQLQ KQTDLMQDTS RLLDPYIRIQ GLDVPKLREH CRERPGAFPS EETLRGLGRR GFLQTLNATL GCVLHRLADL EQRLPKAQDL ERSGLNIEDL EKLQMARPNI LGLRNNIYCM AQLLDNSDTA EPTKAGRGAS QPPTPTPASD AFQRKLEGCR FLHGYHRFMH SVGRVFSKWG ESPNRSRRHS PHQALRKGVR RTRPSRKGKR LMTRGQLPR Endotoxin :
< 0.2 EU/μg, determined by LAL method. Reconstitution :
Reconstituted in ddH2O at 100 μg/mL. Storage :
Lyophilized recombinant human Oncostatin M (rhOSM) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhOSM should be stable up to 2 weeks at 4°C or up to 3 months at -20°C. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description :
Oncostatin M (OSM) is a multifunctional cytokine, and belongs to Interleukin-6 (IL-6) subfamily, including IL-11, leukemia inhibitory factor (LIF), ciliary neurotropic factor, cardiotrophin-1, and novel neurotropin-1. In vivo, OSM is secreted from activated T cells, monocytes, neutrophils, and endothelial cells. OSM is related to LIF, and share a receptor with LIF in human. Human OSM can bind to gp130 and recruit OSM Receptor β or LIF Receptor β to form a ternary complex. OSM stimulates the growth of different types of cells, including megakaryocytes, fibroblasts, vascular endothelial cells, and T cells. On the other hand, OSM inhibits the proliferation of several cancer cell lines, such as solid tissue tumor cells, lung cancer cells, melanoma cells, and breast cancer cells.Recombinant human Oncostatin M (rhOSM) produced in E.coli is a single non-glycosylated polypeptide chain containing 228 amino acids. A fully biologically active molecule, rhOSM has a molecular mass of 25.9 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Blocking peptide available as BK0147-50μgP