Recombinant TNF-α, His, Human 
Datasheet
COA
MSDS
SHIP
Recombinant :
Recombinant TNF-α, His, Human Source :
Escherichia coli. Molecular Weight :
18.3kDa, observed by reducing SDS-PAGE. Purity :
> 95% by SDS-PAGE analysis. Biological Activity :
ED50<0.13ng/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of >7.69 x 10ˆ6units/mg. Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized after extensive dialysis against PBS. AA Sequence :
MHHHHHHVR SSSRTPSDKP VAHVVANPQA EGQLQWLNRR ANALLANGVE LRDNQLVVPS EGLYLIYSQV LFKGQGCPST HVLLTHTISR IAVSYQTKVN LLSAIKSPCQ RETPEGAEAK PWYEPIYLGG VFQLEKGDRL SAEINRPDYL DFAESGQVYF GIIAL Endotoxin :
<1.0 EU/μg, determined by LAL method. Reconstitution :
Reconstituted in ddH2O at 100 μg/ml. Storage :
Lyophilized recombinant human Tumor Necrosis Factor-Alpha (rhTNF-alpha) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhTNF-alpha should be stable up to 4 weeks at 4°C or up to 6 months at -20°C. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description :
Tumor Necrosis Factor-Alpha (TNF-alpha) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.Recombinant human Tumor Necrosis Factor-Alpha (rhTNF-alpha) produced in E. coli is a non-glycosylated polypeptide chain of 164 amino acids. A fully biologically active molecule, rhTNF-alpha has a molecular mass of 18.3kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Recombinant TNF-α, His, Human Source :
Escherichia coli. Molecular Weight :
18.3kDa, observed by reducing SDS-PAGE. Purity :
> 95% by SDS-PAGE analysis. Biological Activity :
ED50<0.13ng/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of >7.69 x 10ˆ6units/mg. Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation :
Lyophilized after extensive dialysis against PBS. AA Sequence :
MHHHHHHVR SSSRTPSDKP VAHVVANPQA EGQLQWLNRR ANALLANGVE LRDNQLVVPS EGLYLIYSQV LFKGQGCPST HVLLTHTISR IAVSYQTKVN LLSAIKSPCQ RETPEGAEAK PWYEPIYLGG VFQLEKGDRL SAEINRPDYL DFAESGQVYF GIIAL Endotoxin :
<1.0 EU/μg, determined by LAL method. Reconstitution :
Reconstituted in ddH2O at 100 μg/ml. Storage :
Lyophilized recombinant human Tumor Necrosis Factor-Alpha (rhTNF-alpha) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhTNF-alpha should be stable up to 4 weeks at 4°C or up to 6 months at -20°C. Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only. Description :
Tumor Necrosis Factor-Alpha (TNF-alpha) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.Recombinant human Tumor Necrosis Factor-Alpha (rhTNF-alpha) produced in E. coli is a non-glycosylated polypeptide chain of 164 amino acids. A fully biologically active molecule, rhTNF-alpha has a molecular mass of 18.3kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Blocking peptide available as BK0169-1mgP