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Recombinant TNF-α, Rhesus Macaque  BK0173-1mg
RecombinantRecombinant TNF-α, Rhesus Macaque 
Catalog No.BK0173-1mg
SourceEscherichia coli.
Molecular Weight17.4 kDa, observed by reducing SDS-PAGE.
Purity> 95% as analyzed by SDS-PAGE& HPLC
Biological ActivityED50 < 60 pg/ml, measured in a cytotoxicity assay using mouse L-929 cells in the presence of actinomycin D, corresponding to a specific activity of > 1.6 × 10ˆ7 units/mg.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationLyophilized after extensive dialysis against PBS
Endotoxin< 0.2 EU/μg, determined by LAL method.
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Recombinant :
Recombinant TNF-α, Rhesus Macaque 
Source :
Escherichia coli.
Molecular Weight :
17.4 kDa, observed by reducing SDS-PAGE.
Purity :
> 95% as analyzed by SDS-PAGE& HPLC
Biological Activity :
ED50 < 60 pg/ml, measured in a cytotoxicity assay using mouse L-929 cells in the presence of actinomycin D, corresponding to a specific activity of > 1.6 × 10ˆ7 units/mg.
Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation :
Lyophilized after extensive dialysis against PBS
AA Sequence :
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL
Endotoxin :
< 0.2 EU/μg, determined by LAL method.
Reconstitution :
Reconstituted in ddH2O at 100 μg/ml.
Storage :
Lyophilized recombinant Rhesus Macaque TNF-α, remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Rhesus Macaque TNF-α should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description :
Tumor Necrosis Factor-Alpha (TNF-α) plays a major role in regulating growth, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells. In addition to inducing hemorrhagic necrosis of tumors, studies indicate TNF is involved in certain types of tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, Crohn's disease, rheumatoid arthritis and graft-versus-host disease.Recombinant Rhesus Macaque TNF-α produced in E.coli is a single, non-glycosylated polypeptide chain containing 157 amino acids. A fully biologically active molecule, Recombinant Rhesus Macaque TNF-α has a molecular mass of 17.4 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Blocking peptide available as BK0173-1mgP
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