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Recombinant PDGF-BB, Human (P. pastoris-expressed)  BK0343-10μg
RecombinantRecombinant PDGF-BB, Human (P. pastoris-expressed) 
Catalog No.BK0343-10μg
SourceP. pastoris
Molecular Weight24.3kDa, observed by non-reducing SDS-PAGE.
Purity> 97% by SDS-PAGE and HPLC analyses.
Biological ActivityED50 <3 ng/ml, measured by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells, corresponding to a specific activity of >3.3 x 10ˆ5 units/mg.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationLyophilized after extensive dialysis against 10mM acetic acid.
Endotoxin<1.0 EU/μg, determined by LAL method.
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Recombinant :
Recombinant PDGF-BB, Human (P. pastoris-expressed) 
Source :
P. pastoris
Molecular Weight :
24.3kDa, observed by non-reducing SDS-PAGE.
Purity :
> 97% by SDS-PAGE and HPLC analyses.
Biological Activity :
ED50 <3 ng/ml, measured by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells, corresponding to a specific activity of >3.3 x 10ˆ5 units/mg.
Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation :
Lyophilized after extensive dialysis against 10mM acetic acid.
AA Sequence :
SL GSLTIAEPAM IAECKTRTEV FEISRRLIDR TNANFLVWPP CVEVQRCSGC CNNRNVQCRP TQVQLRPVQV RKIEIVRKKP IFKKATVTLE DHLACKCETV AAARPVT
Endotoxin :
<1.0 EU/μg, determined by LAL method.
Reconstitution :
Reconstituted in ddH2O at 100 μg/ml.
Storage :
Lyophilized recombinant human Platelet-Derived Growth Factor-BB (rhPDGF-BB) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhPDGF-BB should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.
Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description :
Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle, connective tissue, bone and cartilage cells, and some blood cells. The PDGF is involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. PDGF which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer.Recombinant human Platelet-Derived Growth Factor-BB (rhPDGF-BB) produced in Pichia pastoris is a glycosylated polypeptide. rhPDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids) and is obtained by proprietary chromatographic techniques at GenScript.
Blocking peptide available as BK0343-10μgP
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