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Recombinant TNF-α, Mouse (P. pastoris-expressed)  BK0348-20μg
RecombinantRecombinant TNF-α, Mouse (P. pastoris-expressed) 
Catalog No.BK0348-20μg
SourceP. pastoris
Molecular Weight17kDa, observed by reducing SDS-PAGE.
Purity> 95% as analyzed by reducing SDS-PAGE.
Biological ActivityED50<0.01ng/ml, measured by cytotoxicity assay using L929 cells, corresponding to a specific activity of >1 x 10ˆ8units/mg.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationLyophilized after extensive dialysis against PBS.
Endotoxin<1 EU/μg, determined by LAL method.
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Recombinant :
Recombinant TNF-α, Mouse (P. pastoris-expressed) 
Source :
P. pastoris
Molecular Weight :
17kDa, observed by reducing SDS-PAGE.
Purity :
> 95% as analyzed by reducing SDS-PAGE.
Biological Activity :
ED50<0.01ng/ml, measured by cytotoxicity assay using L929 cells, corresponding to a specific activity of >1 x 10ˆ8units/mg.
Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation :
Lyophilized after extensive dialysis against PBS.
AA Sequence :
SQNSSD KPVAHVVANH QVEEQLEWLS QRANALLANG MDLKDNQLVV PADGLYLVYS QVLFKGQGCP DYVLLTHTVS RFAISYQEKV NLLSAVKSPC PKDTPEGAEL KPWYEPIYLG GVFQLEKGDQ LSAEVNLPKY LDFAESGQVY FGVIAL
Endotoxin :
<1 EU/μg, determined by LAL method.
Reconstitution :
Reconstituted in ddH2O or PBS at 100 μg/ml.
Storage :
Lyophilized recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-α) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmTNF-αshould be stable up to 1 week at 4°C or up to 2 months at -20°C.
Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description :
Sharing 79% sequence identity with human Tumor necrosis factor-alpha (hTNF-α), mouse Tumor Necrosis Factor-alpha (mTNF-α) is a cytokine mainly expressed by immune cells [1]. A type II transmembrane protein, TNF-α is further proteolytically processed to a soluble form [2]. The trimeric active TNF-α[3] then exerts its diverse biological properties including gapoptosis, inflammation, autoimmunity and cell proliferation by binding to TNF Receptor 1 and 2 [4]. Recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-α) produced in P.pastoris is a fully biologically active polypeptide chain of 152 amino acids with a molecular mass of 17 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Blocking peptide available as BK0348-20μgP
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