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Recombinant VEGF165, Human  BK0352-50μg
RecombinantRecombinant VEGF165, Human 
Catalog No.BK0352-50μg
SourceP. pastoris
Molecular Weight 38.2kDa, observed by non-reducing SDS-PAGE
Purity > 95% as analyzed by reducing SDS-PAGE.
Biological ActivityED50 of 1-5ng/ml, measured by the dose-dependent stimulation of the proliferation of HUVEC cells, corresponding to a specific activity of 2x 10ˆ5-1x 10ˆ6 units/mg.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized after extensive dialysis against 25 mM HEPES and 150 mM NaCl, pH 7.0.
Endotoxin<0.5 EU/μg, determined by LAL method.
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Recombinant :
Recombinant VEGF165, Human 
Source :
P. pastoris
Molecular Weight :
38.2kDa, observed by non-reducing SDS-PAGE
Purity :
> 95% as analyzed by reducing SDS-PAGE.
Biological Activity :
ED50 of 1-5ng/ml, measured by the dose-dependent stimulation of the proliferation of HUVEC cells, corresponding to a specific activity of 2x 10ˆ5-1x 10ˆ6 units/mg.
Physical Appearance :
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation :
Lyophilized after extensive dialysis against 25 mM HEPES and 150 mM NaCl, pH 7.0.
AA Sequence :
APMAEG GGQNHHEVVK FMDVYQRSYC HPIETLVDIF QEYPDEIEYI FKPSCVPLMR CGGCCNDEGL ECVPTEESNI TMQIMRIKPH QGQHIGEMSF LQHNKCECRP KKDRARQENP CGPCSERRKH LFVQDPQTCK CSCKNTDSRC KARQLELNER TCRCDKPRR
Endotoxin :
<0.5 EU/μg, determined by LAL method.
Reconstitution :
Reconstituted in ddH2O at 100 μg/ml.
Storage :
Lyophilized recombinant human Vascular Endothelial Growth Factor A165 (rhVEGF-A165) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhVEGF-A165 should be stable up to 4 week at 4°C or up to 6 months at -20°C.
Usage :
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description :
Vascular Endothelial Growth Factor (VEGF) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, Vascular Endothelial Growth Factor (VEGF) plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates Vascular Endothelial Growth Factor (VEGF) in the induction of tumor metastasis and intra-ocular neovascular syndromes. Vascular Endothelial Growth Factor (VEGF) signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product.Recombinant human Vascular Endothelial Growth Factor A165 (rhVEGF-A165) produced in Pichia pastoris is a disulfide-linked homodimer containing two polypeptide chains of 165 amino acids each. A fully biologically active molecule, rhVEGF-A165 has a molecular mass of 38.2kDa analyzed by non-reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Blocking peptide available as BK0352-50μgP
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