Product Name :
TLE1/2/3/4 polyclonal antibody Background :
Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
IgG Immunogen :
The antiserum was produced against synthesized peptide derived from the C-terminal region of human TLE1/TLE2/TLE3/TLE4. AA range:721-770 Conjugate :
Unconjugated Modification :
Unmodified

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  Western blot analysis of TLE1/2/3/4 in A549, 3T3, mouse liver, SH-SY5Y, K562 , Hela , 293T lysates using TLE1/2/3/4 antibody.
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  Immunohistochemistry analysis of paraffin-embedded Human breastcancer using TLE1/2/3/4 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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  Immunohistochemistry analysis of paraffin-embedded Human breastcancer using TLE1/2/3/4 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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  Immunohistochemistry analysis of paraffin-embedded Human breastcancer using TLE1/2/3/4 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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