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Caspase 9 (Tyr153) polyclonal antibody AP0186
  • Western blot (WB) analysis of Caspase 9 (Tyr153) polyclonal antibody at 1:500 dilution Lane1:LO2 whole cell lysate(40ug) Lane2:L02 treated with PBS(1×PBS,PH7.4) for 30 minutes whole cell lysate(40ug) Lane3:L02 treated with PBS(1×PBS,PH7.4) for 120 minutes whole cell lysate(40ug) Lane4:L02 treated with PBS(1×PBS,PH7.4) for 240 minutes whole cell lysate(40ug)
Product NameCaspase 9 (Tyr153) polyclonal antibody
Catalog No.AP0186
Swiss-ProtP55211
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB
Application_allWB: 1:500~1:1000
BiowMW~ 35, 37, 48 kDa
Alternative NameCASP 9; Caspase9; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6; Caspase-9 subunit p35; Caspase-9 subunit p10; CASP9; MCH6
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
Caspase 9 (Tyr153) polyclonal antibody
Background :
Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Caspase 9 (Tyr153) polyclonal antibody detects endogenous levels of full length caspase-9 (48 kDa) and large fragments of caspase-9 (35 kDa and 37kDa).
Immunogen :
Synthetic peptide, corresponding to amino acids 130-180 of Human Caspase 9.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of Caspase 9 (Tyr153) polyclonal antibody at 1:500 dilution Lane1:LO2 whole cell lysate(40ug) Lane2:L02 treated with PBS(1×PBS,PH7.4) for 30 minutes whole cell lysate(40ug) Lane3:L02 treated with PBS(1×PBS,PH7.4) for 120 minutes whole cell lysate(40ug) Lane4:L02 treated with PBS(1×PBS,PH7.4) for 240 minutes whole cell lysate(40ug)
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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