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COL1A1 polyclonal antibody BS60771
  • Western blot (WB) analysis of COL1A1 polyclonal antibody at 1:500 dilution Lane1:MEF whole cell lysate(40ug) Lane2:C6 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:COS-7 whole cell lysate(40ug) Lane5:HCT-116 whole cell lysate(40ug)
Product NameCOL1A1 polyclonal antibody
Catalog No.BS60771
Swiss-ProtP02452
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB
Application_allWB: 1:500~1:1000
BiowMW~ 139 kDa
Alternative NameCollagen alpha-1(I) chain; Alpha-1 type I collagen; COL1A1
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
COL1A1 polyclonal antibody
Background :
The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
COL1A1 polyclonal antibody detects endogenous levels of COL1A1 protein.
Immunogen :
A synthetic peptide corresponding to residues in Human COL1A1
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of COL1A1 polyclonal antibody at 1:500 dilution Lane1:MEF whole cell lysate(40ug) Lane2:C6 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:COS-7 whole cell lysate(40ug) Lane5:HCT-116 whole cell lysate(40ug)
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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