Bioepitope® Bicinchoninic Acid Protein Assay Kit 
Datasheet
COA
MSDS
SHIP
Product Name :
Bioepitope® Bicinchoninic Acid Protein Assay Kit Applications :
1) A&B are stored at RT. There might be needle-crystals in A solution if stored at 4 ℃. It can be continued to use after thawing at RT. That causes no impact on detection effect. 2) Certain substances including reducing potential, chelating agents, and strong acids or bases are known to interfere with protein estimation and avoid those substances in the sample buffer. (For example, EDTA, EGTA, DTT) 3) Prepare a clear and fresh WR reagent at room temperature when prepping new experiments. After addingWR reagent, it could be incubated for sixty minutes at 37°C or 2 hours at room temperature. Absorbance at 562nm increases with the increasing incubation time. Color development runs faster with the increasing temperature. If sample concentration is too low, it will be better to run the reaction at a higher temperature or increase the incubation time. 4) Good linear range for samples is from 50-2000μg/ml. 5) BCA assay is interfered with by chelating agents and high concentration reducing agents. Make sure EDTA<10mM, no EGTA, DTT<1mM and β-ME <1mMin in the sample buffer. Try to remove the interfering substance by dialysis or gel filtration to eliminate or minimize the effects of interfering substances. If interference can not be overcome, it is recommend you use the Bradford protein assay kit. Background :
Protein quantification is often required before precessing protein samples for analysis. The Bicinchoninic Acid (BCA) Protein Assay is highly sensitive colorimetric assay that is not affected by chemicals in the sample. The BCA Protein Assay primarily reduces Cu2+ to Cu1+ by proteins in an alkaline environment followed by highly sensitive and selective colorimetric detection of BCA/copper complex. It is water-soluble and strongly absorbs light at 562nm in a linear fashion with increasing protein concentration. Alternative Name :
Classification :
Protein Related Reagents Format :
Kit Contents :
Biogot BCA Reagent A: 100ml, stored at room temperature Biogot BCA Reagent B: 2ml stored at room temperature Bovine Serum Albumin Standard: 100mg(Powder), Stored at 4℃.( Recommend to make stock solution(2mg/ml) and aliquot before store at -20℃ ) Storage & Shelf Life :
Store at 4° C Procedure :
A) Standard Preparation: Label 9 test tubes with A-I and prepare the standards as indicated below. The diluent used should be the same as used for the protein samples. The following dilutions are suitable for duplicate Standard assays. B) Working Solution Preparation: 1) Use following formula to determine the amount of working solution required. (Total number of standards and samples)*(Number of replicates)*(Volume of working solution sample)= Total volume working solution required. 2) Mix fifty parts of BCA Reagent A with one part of BCA reagent B (50:1, Reagent A:B). C) Micro-plate Procedure (Sample to WR ratio = 1:20) 1. Pipette 10 μl of each standard or unknown sample replicate into a micro-plate well (working range = 20-2,000 μg/ml). 2. Add 200 μl of the WR to each well and mix plate thoroughly on a plate shaker for 30 seconds. 3. Cover plate and incubate in the water bath at 37°C for 30 minutes. 4. Cool plate to RT. 5. Measure the absorbency at or near 562 nm on a plate reader. 6. Use the standard curve to calculate the protein concentration of each unknown sample. Research use :
For research use only, not for use in diagnostic procedures. Expiration Date :
Six months from date of reconstitution.
Bioepitope® Bicinchoninic Acid Protein Assay Kit Applications :
1) A&B are stored at RT. There might be needle-crystals in A solution if stored at 4 ℃. It can be continued to use after thawing at RT. That causes no impact on detection effect. 2) Certain substances including reducing potential, chelating agents, and strong acids or bases are known to interfere with protein estimation and avoid those substances in the sample buffer. (For example, EDTA, EGTA, DTT) 3) Prepare a clear and fresh WR reagent at room temperature when prepping new experiments. After addingWR reagent, it could be incubated for sixty minutes at 37°C or 2 hours at room temperature. Absorbance at 562nm increases with the increasing incubation time. Color development runs faster with the increasing temperature. If sample concentration is too low, it will be better to run the reaction at a higher temperature or increase the incubation time. 4) Good linear range for samples is from 50-2000μg/ml. 5) BCA assay is interfered with by chelating agents and high concentration reducing agents. Make sure EDTA<10mM, no EGTA, DTT<1mM and β-ME <1mMin in the sample buffer. Try to remove the interfering substance by dialysis or gel filtration to eliminate or minimize the effects of interfering substances. If interference can not be overcome, it is recommend you use the Bradford protein assay kit. Background :
Protein quantification is often required before precessing protein samples for analysis. The Bicinchoninic Acid (BCA) Protein Assay is highly sensitive colorimetric assay that is not affected by chemicals in the sample. The BCA Protein Assay primarily reduces Cu2+ to Cu1+ by proteins in an alkaline environment followed by highly sensitive and selective colorimetric detection of BCA/copper complex. It is water-soluble and strongly absorbs light at 562nm in a linear fashion with increasing protein concentration. Alternative Name :
Classification :
Protein Related Reagents Format :
Kit Contents :
Biogot BCA Reagent A: 100ml, stored at room temperature Biogot BCA Reagent B: 2ml stored at room temperature Bovine Serum Albumin Standard: 100mg(Powder), Stored at 4℃.( Recommend to make stock solution(2mg/ml) and aliquot before store at -20℃ ) Storage & Shelf Life :
Store at 4° C Procedure :
A) Standard Preparation: Label 9 test tubes with A-I and prepare the standards as indicated below. The diluent used should be the same as used for the protein samples. The following dilutions are suitable for duplicate Standard assays. B) Working Solution Preparation: 1) Use following formula to determine the amount of working solution required. (Total number of standards and samples)*(Number of replicates)*(Volume of working solution sample)= Total volume working solution required. 2) Mix fifty parts of BCA Reagent A with one part of BCA reagent B (50:1, Reagent A:B). C) Micro-plate Procedure (Sample to WR ratio = 1:20) 1. Pipette 10 μl of each standard or unknown sample replicate into a micro-plate well (working range = 20-2,000 μg/ml). 2. Add 200 μl of the WR to each well and mix plate thoroughly on a plate shaker for 30 seconds. 3. Cover plate and incubate in the water bath at 37°C for 30 minutes. 4. Cool plate to RT. 5. Measure the absorbency at or near 562 nm on a plate reader. 6. Use the standard curve to calculate the protein concentration of each unknown sample. Research use :
For research use only, not for use in diagnostic procedures. Expiration Date :
Six months from date of reconstitution.
Multiple gold nanorods@hierarchically porous silica nanospheres for efficient multi-drug delivery and photothermal therapy
PMCID: Pubmed No.:32263936
Regulation of gonadotropin-releasing hormone (GnRH) receptor-I expression in the pituitary and ovary by a GnRH agonist and antagonist
PMCID: Pubmed No.:19861704
Regulation of AMH and SCF expression in human granulosa cells by GnRH agonist and antagonist
PMCID: Pubmed No.:21699083
Exosomes derived from human bone marrow mesenchymal stem cells promote tumor growth in vivo
PMCID: Pubmed No.:22055459
Crocetin induces apoptosis of BGC-823 human gastric cancer cells
PMCID: Pubmed No.:24337515
Effect of Tenuifoliside A isolated from Polygala tenuifolia on the ERK and PI3K pathways in C6 glioma cells.
PMCID: Pubmed No.:24877714
Removal of milk fat globules from whey protein concentrate 34% to prepare clear and heat-stable protein dispersions
PMCID: Pubmed No.:25108870
Tetrandrine Suppresses Lipopolysaccharide-Induced Microglial Activation by Inhibiting NF-κB and ERK Signaling Pathways in BV2 Cells
PMCID: Pubmed No.:25115855
AG4, a compound isolated from Radix Ardisiae Gigantifoliae, induces apoptosis in human nasopharyngeal cancer CNE cells through intrinsic and extrinsic apoptosis pathways.
PMCID: Pubmed No.:25521557
Silencing of SIAH1 in SH-SY5Y affects α-synuclein degradation pathway
PMCID: Pubmed No.:26722480
Removal of milk fat globules from whey protein concentrate 34% to prepare clear and heat-stable protein dispersions
PMCID: Pubmed No.:25108870
Tetrandrine Suppresses Lipopolysaccharide-Induced Microglial Activation by Inhibiting NF-κB and ERK Signaling Pathways in BV2 Cells
PMCID: Pubmed No.:25115855
AG4, a compound isolated from Radix Ardisiae Gigantifoliae, induces apoptosis in human nasopharyngeal cancer CNE cells through intrinsic and extrinsic apoptosis pathways.
PMCID: Pubmed No.:25521557
Silencing of SIAH1 in SH-SY5Y affects α-synuclein degradation pathway
PMCID: Pubmed No.:26722480
Crocetin induces apoptosis of BGC-823 human gastric cancer cells
PMCID: Pubmed No.:24337515
Protective effects of linalool against amyloid beta-induced cognitive deficits and damages in mice
PMCID: Pubmed No.:28235543
Maresin 1 Mitigates High Glucose-Induced Mouse Glomerular Mesangial Cell Injury by Inhibiting Inflammation and Fibrosis
PMCID: Pubmed No.:28182085
Hephaestin and ceruloplasmin facilitate iron metabolism in the mouse kidney
PMCID: Pubmed No.:27991585
Effects of dietary methionine on breast muscle growth, myogenic gene expression and IGF-I signaling in fast- and slow-growing broilers
PMCID: Pubmed No.:28507342
Expression and inhibitory role of TIMP-3 in hepatocellular carcinoma
PMCID: Pubmed No.:27222429
Human Umbilical Cord Mesenchymal Stem Cells Protect Against SCA3 by Modulating the Level of 70 kD Heat Shock Protein
PMCID: Pubmed No.:28667374
Orientin alleviates cognitive deficits and oxidative stress in Aβ1–42-induced mouse model of Alzheimer's disease
PMCID: Pubmed No.:25497709
Maternal exposure to fine particulate air pollution induces epithelial-to-mesenchymal transition resulting in postnatal pulmonary dysfunction mediated by transforming growth factor-β/Smad3 signaling
PMCID: Pubmed No.:28041981
PSD-93 Attenuates Amyloid-β-Mediated Cognitive Dysfunction by Promoting the Catabolism of Amyloid-β
PMCID: Pubmed No.:28697571
Phosphorylated heat shock protein 27 promotes lipid clearance in hepatic cells through interacting with STAT3 and activating autophagy
PMCID: Pubmed No.:27185187
Papaverine inhibits lipopolysaccharide-induced microglial activation by suppressing NF-κB signaling pathway
PMCID: Pubmed No.:27013863
Daphnetin reduces endotoxin lethality in mice and decreases LPS-induced inflammation in Raw264.7 cells via suppressing JAK/STATs activation and ROS production
PMCID: Pubmed No.:28409189
Oridonin Attenuates Synaptic Loss and Cognitive Deficits in an Aβ1–42-Induced Mouse Model of Alzheimer’s Disease
PMCID: Pubmed No.:26974541
Tctex1 plays a key role in the α-synuclein autophagy lysosomal degradation pathway
PMCID: Pubmed No.:28970129
Neurodegenerative changes and neuroapoptosis induced by systemic lipopolysaccharide administration are reversed by dexmedetomidine treatment in mice
PMCID: Pubmed No.:28173746
Bcl‑2 associated athanogene 4 promotes proliferation, migration and invasion of gastric cancer cells
PMCID: Pubmed No.:29067445
Anti-depressant-like effects of Jieyu chufan capsules in a mouse model of unpredictable chronic mild stress
PMCID: Pubmed No.:28810562
Silencing of SIAH1 in SH-SY5Y affects α-synuclein degradation pathway
PMCID: Pubmed No.:26722480
Evaluation of sulfane sulfur bioeffects via a mitochondria-targeting selenium-containing near-infrared fluorescent probe
PMCID: Pubmed No.:29348054
Glutathione Peroxidase-Activatable Two-Photon Ratiometric Fluorescent Probe for Redox Mechanism Research in Aging and Mercury Exposure Mice Models
PMCID: Pubmed No.:31858778
Ratiometric Near-Infrared Fluorescent Probe for Synergistic Detection of Monoamine Oxidase B and Its Contribution to Oxidative Stress in Cell and Mice Aging Models
PMCID: Pubmed No.:29400049
Tannins Enriched Fraction of Emblica officinalis Fruits Alleviates High-Salt and Cholesterol Diet-Induced Cognitive Impairment in Rats via Nrf2–ARE Pathway
PMCID: Pubmed No.:29441016
Timosaponin AIII Induces G2/M Arrest and Apoptosis in Breast Cancer by Activating the ATM/Chk2 and p38 MAPK Signaling Pathways
PMCID: Pubmed No.:33628174
Hypercapnia induces IL-1β overproduction via activation of NLRP3 inflammasome: implication in cognitive impairment in hypoxemic adult rats
PMCID: Pubmed No.:29304864
Ethanol Alleviates Amyloid-β-Induced Toxicity in an Alzheimer’s Disease Model of Caenorhabiditis elegans
PMCID: Pubmed No.:34867289
Protective Effect of JXT Ethanol Extract on Radiation-Induced Hematopoietic Alteration and Oxidative Stress in the Liver
PMCID: Pubmed No.:30510630
Rosuvastatin alleviates high-salt and cholesterol diet-induced cognitive impairment in rats via Nrf2–ARE pathway
PMCID: Pubmed No.:29961403
Sodium butyrate supplementation ameliorates diabetic inflammation in db/db mice
PMCID: Pubmed No.:29941502
Muscone Ameliorates Synaptic Dysfunction and Cognitive Deficits in APP/PS1 Mice
PMCID: Pubmed No.:32538849
Curcumin restrains hepatic glucose production by blocking cAMP/PKA signaling and reducing acetyl CoA accumulation in high-fat diet (HFD)-fed mice
PMCID: Pubmed No.:29499209
Deletion of hephaestin and ceruloplasmin induces a serious systemic iron deficiency and disrupts iron homeostasis
PMCID: Pubmed No.:30060949
RIPK2-Mediated Autophagy and Negatively Regulated ROS-NLRP3 Inflammasome Signaling in GMCs Stimulated with High Glucose
PMCID: Pubmed No.:31485193
Dexmedetomidine protects hippocampal neurons against hypoxia/reoxygenation-induced apoptosis through activation HIF-1α/p53 signaling
PMCID: Pubmed No.:31260683
MIR22HG Regulates the Proliferation, Epithelial-Mesenchymal Transition, and Apoptosis in Colorectal Carcinoma
PMCID: Pubmed No.:33493419
Hypertonic saline downregulates endothelial cell-derived VEGF expression and reduces blood-brain barrier permeability induced by cerebral ischaemia via the VEGFR2/eNOS pathway
PMCID: Pubmed No.:31524227
Huatuo Zaizao pill ameliorates cognitive impairment of APP/PS1 transgenic mice by improving synaptic plasticity and reducing Aβ deposition
PMCID: Pubmed No.:29843688
Estrogen improved the regeneration of axons after subcortical axon injury via regulation of PI3K/Akt/CDK5/Tau pathway
PMCID: Pubmed No.:32755041
Betaine improves the growth performance and muscle growth of partridge shank broiler chickens via altering myogenic gene expression and insulin-like growth factor-1 signaling pathway
PMCID: Pubmed No.:30085311
Optimized adipose tissue engineering strategy based on a neo‐mechanical processing method
PMCID: Pubmed No.:29802722
Interleukin‑1 induces receptor activator of nuclear factor‑κB ligand‑independent osteoclast differentiation in RAW264.7 cells
PMCID: Pubmed No.:33968171
miR-181a targets GATA6 to inhibit the progression of human laryngeal squamous cell carcinoma
PMCID: Pubmed No.:29517349
Inhibition of inducible nitric oxide synthase improved erectile dysfunction in rats with type 1 diabetes
PMCID: Pubmed No.:34137064
Effect of bone marrow mesenchymal stem cells on the polarization of macrophages
PMCID: Pubmed No.:29363724
Silencing of Tctex1 impairs autophagy lysosomal degradation of α-synuclein and cell viability
PMCID: Pubmed No.:29406369
Ablation of the MiR-17-92 MicroRNA Cluster in Germ Cells Causes Subfertility in Female Mice
PMCID: Pubmed No.:29402772
Enhanced NCLX-dependent mitochondrial Ca2+ efflux attenuates pathological remodeling in heart failure
PMCID: Pubmed No.:35358843
Recombinant humanized IgG1 maintain liver triglyceride homeostasis through Arylacetamide deacetylase in ApoE−/− mice
PMCID: Pubmed No.:35397394
A study of diabetes-induced erectile dysfunction treated with human umbilical cord mesenchymal stem cells
PMCID: Pubmed No.:35415927
PELP1 is overexpressed in lung cancer and promotes tumor cell malignancy and resistance to tyrosine kinase inhibitor drug
PMCID: Pubmed No.:35969940
Blocking peptide available as BD0028P