Super Sensitive TM IHC Detection System kit （Mouse/Rabbit） 
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Product Name :
Super Sensitive TM IHC Detection System kit （Mouse/Rabbit） Applications :
Super Sensitive Detection System detects mouse or rabbit antibody；It can apply for paraffin-embedded tissue, cryostat sections, blood smears, and cell preparations. Background :
Super Sensitive Detection System kit is the latest technology in polymeric labeling. Polymer detection methods have been shown to provide increased sensitivity. This innovative polymer technology has major advantages than conventional IHC systems. Super Sensitive Detection System amplifies the signal with both mouse and rabbit primary antibodies. Background noise due to nonspecific binding to endogenous biotin molecules is eliminated because Super Sensitive Detection is not a biotin/avidin based system, which eliminates the background noise due to nonspecific binding to endogenous biotin. The Super Sensitive Detection System provides the user with a rapid, easy to use, and versatile IHC detection system. Alternative Name :
1. Within the validity period; 2. Not intended to clinical diagnosis. Classification :
IHC Related Reagents Format :
Kit Contents :
A:Hydrogen Peroxide Blocking ReagentB:Blocking ReagentC:Antibody AmplifierD:HRP PolymerE:DAB Substrate ReagentF:DAB Chromogen Reagent Storage & Shelf Life :
Store at 2-8°C. Each component is stable for up to 12 Months Procedure :
1. Deparaffinize and rehydrate tissue section；PBS/TBS wash for 2 min×3； 2. Incubate tissue in appropriate pretreatment or digestive enzyme if required for primary antibody; and PBS/TBS wash for 2 min×3; 3. Incubate slide in Hydrogen Peroxide Blocking Reagent for 10 minutes, PBS/TBS wash for 2 min×3; 4. Apply Blocking Reagent and incubate for 5 minutes, PBS/TBS wash for 2 min×3; (May be omitted if primary antibodies are diluted in buffers containing normal goat serum.); 5. Apply primary antibody and incubate according to manufacturer\'s recommended protocol，PBS/TBS wash for 2 min×3； 6. Apply HRP Polymer and incubate for 10 min; (NOTE: HRP is light sensitive. Please avoid unnecessary light exposure.)，PBS/TBS wash for 2 min×3； 7. Add 30 μl (1 drop) DAB Chromogen to 1 ml of DAB Substrate, mix by swirling and apply to tissue. Incubate for about 3-5 minutes，PBS/TBS wash for 2 min×3； 8. Counterstain and coverslip using a permanent mounting media. Research use :
For research use only, not for use in diagnostic procedures. Expiration Date :
Super Sensitive TM IHC Detection System kit （Mouse/Rabbit） Applications :
Super Sensitive Detection System detects mouse or rabbit antibody；It can apply for paraffin-embedded tissue, cryostat sections, blood smears, and cell preparations. Background :
Super Sensitive Detection System kit is the latest technology in polymeric labeling. Polymer detection methods have been shown to provide increased sensitivity. This innovative polymer technology has major advantages than conventional IHC systems. Super Sensitive Detection System amplifies the signal with both mouse and rabbit primary antibodies. Background noise due to nonspecific binding to endogenous biotin molecules is eliminated because Super Sensitive Detection is not a biotin/avidin based system, which eliminates the background noise due to nonspecific binding to endogenous biotin. The Super Sensitive Detection System provides the user with a rapid, easy to use, and versatile IHC detection system. Alternative Name :
1. Within the validity period; 2. Not intended to clinical diagnosis. Classification :
IHC Related Reagents Format :
Kit Contents :
A:Hydrogen Peroxide Blocking ReagentB:Blocking ReagentC:Antibody AmplifierD:HRP PolymerE:DAB Substrate ReagentF:DAB Chromogen Reagent Storage & Shelf Life :
Store at 2-8°C. Each component is stable for up to 12 Months Procedure :
1. Deparaffinize and rehydrate tissue section；PBS/TBS wash for 2 min×3； 2. Incubate tissue in appropriate pretreatment or digestive enzyme if required for primary antibody; and PBS/TBS wash for 2 min×3; 3. Incubate slide in Hydrogen Peroxide Blocking Reagent for 10 minutes, PBS/TBS wash for 2 min×3; 4. Apply Blocking Reagent and incubate for 5 minutes, PBS/TBS wash for 2 min×3; (May be omitted if primary antibodies are diluted in buffers containing normal goat serum.); 5. Apply primary antibody and incubate according to manufacturer\'s recommended protocol，PBS/TBS wash for 2 min×3； 6. Apply HRP Polymer and incubate for 10 min; (NOTE: HRP is light sensitive. Please avoid unnecessary light exposure.)，PBS/TBS wash for 2 min×3； 7. Add 30 μl (1 drop) DAB Chromogen to 1 ml of DAB Substrate, mix by swirling and apply to tissue. Incubate for about 3-5 minutes，PBS/TBS wash for 2 min×3； 8. Counterstain and coverslip using a permanent mounting media. Research use :
For research use only, not for use in diagnostic procedures. Expiration Date :
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