Contact us :
Home > Product > Research Reagents
BiodlightTM ECL Chemiluminescent HRP Substrate (High Sensitivity) BLH01S020
Product NameBiodlightTM ECL Chemiluminescent HRP Substrate (High Sensitivity)
Catalog No.BLH01S020
ClassificationWB Related Reagents
ContentsLuminol Reagent,10ml Peroxide Solution,10ml
Storage & Shelf LifeStore at 4° C
Research useFor research use only, not for use in diagnostic procedures.
Expiration DateOne year from date of reconstitution.
Browse similar products>>
Size Price
10+10ml $46
Add to cart My orders
Product Name :
BiodlightTM ECL Chemiluminescent HRP Substrate (High Sensitivity)
Applications :
Western Blot
Background :
BiodlightTM ECL Chemiluminescent HRP Substrate provides high sensitivity in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose transfer membranes,and is compatible with all commonly used buffers and blocking reagents.The HRP substrate consists of Luminol Reagent and Perixide Solution.Working HRP substrate is prepared by combining equal volumes of Luminol Reagent and Perixide Solution.The HRP substrate produces a high intensity signal with low background for detection of both high and low abundance.
Alternative Name :
Classification :
WB Related Reagents
Format :
Contents :
Luminol Reagent,10ml Peroxide Solution,10ml
Storage & Shelf Life :
Store at 4° C
Procedure :
1. Remove blot from the transfer apparatus and block nonspecific sites with Blocking Reagent for 20-60 minutes at room temperature (RT) with shaking. For best results, block for 1 hour at RT. 2. Remove the Blocking Reagent and add the appropriate primary antibody dilution. Incubate blot for 1 hour with shaking.If desired, blots may be incubated with primary antibody overnight at 2-8°C. 3. Wash membrane by suspending it in Wash Buffer and agitating for 5 minutes. Replace Wash Buffer at least 4-6 times. Increasing the wash buffer volume and/or the number of washes may help reduce background.Briefly rinsing membrane in wash buffer before incubation will increase wash efficiency. 4.Incubate blot with the appropriate HRP-conjugate dilution for 1 hour at RT with shaking. 5.Repeat Step 3 to remove non-bound HRP-conjugate.Membrane must be thoroughly washed after incubation with the HRP-conjugate. 6.Prepare Working Solution by mixing equal parts of the Stable Peroxide Solution and the Luminol/Enhancer Solution. Use 0.1 ml Working Solution per cm2 of membrane. The Working Solution is stable for 8 hours at room temperature.Exposure to the sun or any other intense light can harm the Working Solution. For best results keep the Working Solution in an amber bottle and avoid prolonged exposure to any intense light. Typical laboratory lighting will not harm the Working Solution. 7.Incubate blot with Working Solution for 5 minutes. 8.Remove blot from Working Solution and place it in a plastic membrane protector; a plastic sheet protector or plastic wrap may be used. Use an absorbent tissue to remove excess liquid and to carefully press out any bubbles from between the blot and surface of the membrane protector. 9.Place the protected membrane in a film cassette with the protein side facing up. Turn off all lights except those appropriate for film exposure (e.g., a red safelight). Film must remain dry during exposure. For optimal results, perform the following precautions: • Make sure excess substrate is removed from the membrane and the membrane protector. • Use gloves during the entire film-handling process. • Never place a blot on developed film, as there may be chemicals on the film that will reduce signal. 10.Carefully place a piece of film on top of the membrane. A recommended first exposure time is 60 seconds. Exposure time may be varied to achieve optimal results. Enhanced or pre-flashed film is not necessary.Light emission is intense and any movement between the film and membrane can cause artifacts on the film.The exposure time may be varied to achieve optimal results. If the signal is too intense, reduce exposure time or optimize the system by decreasing the antigen and/or antibody concentrations.Light emission is most intense during the first 5-30 minutes after substrate incubation. Light emission will continue for several hours, but will decrease with time. Longer exposure times may be necessary as the blot ages. 11.Develop film using appropriate developing solution and fixative. Blot may be stripped and reprobed if necessary.
Research use :
For research use only, not for use in diagnostic procedures.
Expiration Date :
One year from date of reconstitution.
Integral pharmacokinetics of multiple lignan components in normal, CCl4-induced hepatic injury and hepatoprotective agents pretreated rat sand correlations with hepatic injury biomarkers PMCID:    Pubmed No.:20600750 prag01, a novel deltamethrin-resistance-associated gene from Culex pipiens pallens. PMCID:    Pubmed No.:20922424 Protein Kinase C Promotes NMDA Receptor Trafficking by Indirectly Triggering CaMKII Autophosphorylation PMCID:    Pubmed No.:21606495 Thioacetamide Intoxication Triggers Transcriptional Upregulation but Enzyme Inactivation of UDP-glucuronosyltransferases PMCID:    Pubmed No.:21733883 Opsin3 sensitizes hepatocellular carcinoma cells to 5-fluorouracil treatment by regulating the apoptotic pathway PMCID:    Pubmed No.:22313545 Gleditsioside B, a triterpene saponin isolated from the anomalous fruits of Gleditsia sinensis Lam., abrogates bFGF-induced endothelial cell migration through preventing the activation of MMP-2 and FAK via inhibiting ERK and PI3K/AKT signaling pathways PMCID:    Pubmed No.:23026290 Human Umbilical Cord Stem Cells Ameliorate Experimental Autoimmune Encephalomyelitis by Regulating Immunoinflammation and Remyelination PMCID:    Pubmed No.:23140594 Ribose-phosphate pyrophosphokinase 1 (PRPS1) associated with deltamethrin resistance in Culex pipiens pallens PMCID:    Pubmed No.:23250545 Modulation of A-type K+ channels by the short-chain cobrotoxin through the protein kinase C-delta isoform decreases membrane excitability in dorsal root ganglion neurons PMCID:    Pubmed No.:23435353
Blocking peptide available as BLH01S020P
COPYRIGHT © 2015-2018 Bioworld Technology, Inc. All rights reserved POLYCLONAL AND MONOCLONAL ANTIBODY CENTER