Prestained Protein Ladder (10~180 kDa) 
Datasheet
COA
MSDS
SHIP
Product Name :
Prestained Protein Ladder (10~180 kDa) Applications :
• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. • 2~3 μl per well for general Western transfering. • Apply more for thicker (>1.5 mm) or larger gel. Background :
The Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris glycine buffer). The Prestained Protein Ladder is designed for monitoring protein separated during SDS polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading. Alternative Name :
The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition. Classification :
WB Related Reagents Format :
Liquid Contents :
Approximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25°C), 2% SDS, 1 mM 2-Mercaptoethanol, 3.6 M Urea, and 15% (v/v) Glycerol). Storage & Shelf Life :
Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. For long term storage, store at -20°C. Procedure :
User-optimized Research use :
For research use only, not for use in diagnostic procedures. Expiration Date :
Prestained Protein Ladder (10~180 kDa) Applications :
• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. • 2~3 μl per well for general Western transfering. • Apply more for thicker (>1.5 mm) or larger gel. Background :
The Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris glycine buffer). The Prestained Protein Ladder is designed for monitoring protein separated during SDS polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading. Alternative Name :
The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition. Classification :
WB Related Reagents Format :
Liquid Contents :
Approximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25°C), 2% SDS, 1 mM 2-Mercaptoethanol, 3.6 M Urea, and 15% (v/v) Glycerol). Storage & Shelf Life :
Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. For long term storage, store at -20°C. Procedure :
User-optimized Research use :
For research use only, not for use in diagnostic procedures. Expiration Date :
Blocking peptide available as BD0026P