Super Vision FITC Immunofluorescence (IF) Detection System kit (Rabbit Anti-Sheep) 
Datasheet
COA
MSDS
SHIP
Product Name :
Super Vision FITC Immunofluorescence (IF) Detection System kit (Rabbit Anti-Sheep) Applications :
Super Vision FITC IF kit (Rabbit Anti-Sheep) is suitable for use with sheep primary antibodies. It can apply for paraffin-embedded tissue and cryostat sections. Background :
Super Vision FITC Immunofluorescence (IF) Detection System is a non-biotin, one-step detection system that allows for the demonstration of antigens in paraffin-embedded tissue, cryostat sections, and cell preparations. This kit has been developed using a proprietary hyper labeling technology used to label IgG directly with more fluorescein isothiocyanate (FITC). One step polymer system provides increased sensitivity, time savings and detection simplicity. All the components contain with PBS, proteins, stabilizers and preservatives. This kit is suitable for single, double and triple immunofluorescence detect. The color is emerald green with a correct result. Alternative Name :
1. The FITC is easily faded with light; all experiment process need keep away from light; 2. The slide can be stored in 4℃ for about 1 week; and the signal becomes decrease for long time; 3. For research only, not intended to clinical diagnosis. Classification :
IHC Related Reagents Format :
Kit Contents :
A:Blocking Reagent 10ml B:Antibody Solution Buffer 10ml C:Anti-Sheep IgG-FITC 50ul D:Anti-Fading Buffer 10ml Storage & Shelf Life :
Store at 2-8°C. Each component is stable for up to 12 Months. Procedure :
1. Deparaffinize and rehydrate tissue section；PBS/TBS wash for 2 min×3; 2. Incubate tissue in appropriate pretreatment or digestive enzyme if required for primary antibody; and PBS/TBS wash for 2 min×3; 3. Apply Blocking Reagent and incubate for 5 minutes, PBS/TBS wash for 2 min×3 (May be omitted if primary antibodies are diluted in buffers containing normal sheep serum) ; 4. Apply primary antibody and incubate according to manufacturer's recommended protocol，PBS/TBS wash for 2 min×3； 5. Add 5ml-10ml Anti-Sheep IgG-FITC to 1ml Antibody Solution Buffer and vibrate; then add that antibody solution to the sections and incubate for 30-60 min; (NOTE: FITC is light sensitive. Please avoid unnecessary light exposure.)，PBS/TBS wash for 2 min×3； 6. Apply the Anti-Fading Buffer, and cover the microscope cover glass for observation (NOTE: It’s better for observation and operation when the section is dried in no dark box before adding anti-fading buffer). Research use :
For research use only, not for use in diagnostic procedures. Expiration Date :
Super Vision FITC Immunofluorescence (IF) Detection System kit (Rabbit Anti-Sheep) Applications :
Super Vision FITC IF kit (Rabbit Anti-Sheep) is suitable for use with sheep primary antibodies. It can apply for paraffin-embedded tissue and cryostat sections. Background :
Super Vision FITC Immunofluorescence (IF) Detection System is a non-biotin, one-step detection system that allows for the demonstration of antigens in paraffin-embedded tissue, cryostat sections, and cell preparations. This kit has been developed using a proprietary hyper labeling technology used to label IgG directly with more fluorescein isothiocyanate (FITC). One step polymer system provides increased sensitivity, time savings and detection simplicity. All the components contain with PBS, proteins, stabilizers and preservatives. This kit is suitable for single, double and triple immunofluorescence detect. The color is emerald green with a correct result. Alternative Name :
1. The FITC is easily faded with light; all experiment process need keep away from light; 2. The slide can be stored in 4℃ for about 1 week; and the signal becomes decrease for long time; 3. For research only, not intended to clinical diagnosis. Classification :
IHC Related Reagents Format :
Kit Contents :
A:Blocking Reagent 10ml B:Antibody Solution Buffer 10ml C:Anti-Sheep IgG-FITC 50ul D:Anti-Fading Buffer 10ml Storage & Shelf Life :
Store at 2-8°C. Each component is stable for up to 12 Months. Procedure :
1. Deparaffinize and rehydrate tissue section；PBS/TBS wash for 2 min×3; 2. Incubate tissue in appropriate pretreatment or digestive enzyme if required for primary antibody; and PBS/TBS wash for 2 min×3; 3. Apply Blocking Reagent and incubate for 5 minutes, PBS/TBS wash for 2 min×3 (May be omitted if primary antibodies are diluted in buffers containing normal sheep serum) ; 4. Apply primary antibody and incubate according to manufacturer's recommended protocol，PBS/TBS wash for 2 min×3； 5. Add 5ml-10ml Anti-Sheep IgG-FITC to 1ml Antibody Solution Buffer and vibrate; then add that antibody solution to the sections and incubate for 30-60 min; (NOTE: FITC is light sensitive. Please avoid unnecessary light exposure.)，PBS/TBS wash for 2 min×3； 6. Apply the Anti-Fading Buffer, and cover the microscope cover glass for observation (NOTE: It’s better for observation and operation when the section is dried in no dark box before adding anti-fading buffer). Research use :
For research use only, not for use in diagnostic procedures. Expiration Date :
Blocking peptide available as BD5008P