Product Name :
Propidium Iodide（PI）Staining Kit Applications :
This product is 1mg/ml chromogen. Solute it with suitable density for applies. Recommend density is 5ug/ml. Background :
Propidium iodide (PI) has been widely used as a fluorescent stain for DNA in cells; it specificity binds double-stranded nucleic acids in apoptosis, necrosis and fixed cells. PI and DNA complex show light red flourescent color with excitation light 493 nm and emission light 630 nm. It can not throughout the normal living cells membrane, but it enters the apoptosis, necrosis and fixed cells and binds double-stranded DNA. Alternative Name :
1. PI is a possible carcinogen. Operate it with gloves; 2. The PI is faded with light; all experiment process need keep away from light; Classification :
IHC Related Reagents Format :
Kit Contents :
A:Propidium iodide (PI) Chromogen (1mg/ml) 0.1 ml B:Dilution Buffer 20ml Storage & Shelf Life :
Store at 2-8°C; the chromogen store at -20°C. Each component is stable for up to 12 Months. Procedure :
1. For double or triple fluorescence staining in immunofluorescence tests, the PI staining is the last step after all fluorescence antibodies incubation; 2. For culture cell, add 5 ul Chromogen to 10 ml Dilution Buffer in tube and mix it (the end density is 0.5ug/ml); incubate about 5min in a dark incubator, at 30℃; and then wash it with PBS/TBS for 3min×3 times; 3. Observation with the fluorescence microscope. Research use :
For research use only, not for use in diagnostic procedures. Expiration Date :

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Blocking peptide available as BD5012P