Hoechst 33342/ PI Double Staining Kit 
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COA
MSDS
SHIP
Product Name :
Hoechst 33342/ PI Double Staining Kit Applications :
This product is 1mg/ml chromogen. Solute it with suitable density for applies. The recommend density is 5ug/ml. Background :
Hoechst 33342 is a cell permeable fluorescent minor groove-binding probe for DNA, and through the living cell membrane. The Hoechst 33342 and DNA complex show light blue flourescent color. Propidium iodide (PI) has been widely used as a fluorescent stain for DNA in cells; it specificity binds double-stranded nucleic acids in apoptosis, necrosis and fixed cells, but can not enter the normal living cell. PI and DNA complex show light red flourescent color. Hoechst 33342 and PI have been used for double fluorescence staining. Alternative Name :
1. Hoechst 33342/PI are possible carcinogens. Operate them with gloves; 2. The Hoechst 33342/PI are faded with light; all experiment process need keep away from light; Classification :
IHC Related Reagents Format :
Kit Contents :
A:Hoechst 33342/ PI Chromogen (1mg/ml) 0.1 ml B:Dilution Buffer 20ml Storage & Shelf Life :
Store at 2-8°C; the chromogen store at -20°C. Each component is stable for up to 12 Months. Procedure :
1. For double or triple fluorescence staining in immunofluorescence tests, the Hoechst 33342/PI staining is the last step after all fluorescence antibodies incubation; 2. For culture cell, add 10 ul Hoechst 33342/PI Chromogen equally to 10 ml Dilution Buffer in the same tube and mix them (the end density is 0.5ug/ml); incubate about 5min in a dark incubator, at 30℃; and then wash it with PBS/TBS for 3min×3 times; 3. Observation with the fluorescence microscope. Research use :
For research use only, not for use in diagnostic procedures. Expiration Date :
Hoechst 33342/ PI Double Staining Kit Applications :
This product is 1mg/ml chromogen. Solute it with suitable density for applies. The recommend density is 5ug/ml. Background :
Hoechst 33342 is a cell permeable fluorescent minor groove-binding probe for DNA, and through the living cell membrane. The Hoechst 33342 and DNA complex show light blue flourescent color. Propidium iodide (PI) has been widely used as a fluorescent stain for DNA in cells; it specificity binds double-stranded nucleic acids in apoptosis, necrosis and fixed cells, but can not enter the normal living cell. PI and DNA complex show light red flourescent color. Hoechst 33342 and PI have been used for double fluorescence staining. Alternative Name :
1. Hoechst 33342/PI are possible carcinogens. Operate them with gloves; 2. The Hoechst 33342/PI are faded with light; all experiment process need keep away from light; Classification :
IHC Related Reagents Format :
Kit Contents :
A:Hoechst 33342/ PI Chromogen (1mg/ml) 0.1 ml B:Dilution Buffer 20ml Storage & Shelf Life :
Store at 2-8°C; the chromogen store at -20°C. Each component is stable for up to 12 Months. Procedure :
1. For double or triple fluorescence staining in immunofluorescence tests, the Hoechst 33342/PI staining is the last step after all fluorescence antibodies incubation; 2. For culture cell, add 10 ul Hoechst 33342/PI Chromogen equally to 10 ml Dilution Buffer in the same tube and mix them (the end density is 0.5ug/ml); incubate about 5min in a dark incubator, at 30℃; and then wash it with PBS/TBS for 3min×3 times; 3. Observation with the fluorescence microscope. Research use :
For research use only, not for use in diagnostic procedures. Expiration Date :
Blocking peptide available as BD5013P