Product Name :
DAB Powder Applications :
Used for IHC and WB Color reaction. Background :
DAB is utilized in many applications for visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide; the oxidized DAB forms an insoluble brown sediment. The solutions of DAB should be made immediately prior to use for the best results. The literature indicates that it is possible to store solutions or chromogen of DAB for several weeks at -20°C. Report indicated DAB dissolved in Tris buffered saline (pH 7.6) at 0.6 mg/ml can be stable for several weeks at 4°C. Alternative Name :
DAB is a possible carcinogen. Operate it with gloves. Classification :
IHC Related Reagents Format :
Powder Contents :
DAB Powder 1g Storage & Shelf Life :
Store at RT. For 12 Months. Procedure :
1. Dissolve 10 mg in 15 ml of PBS or TBS buffer; 2. Add 12 uL of fresh 30% hydrogen peroxide (or 120 mL fresh 3% hydrogen peroxide) prior to use. Research use :
For research use only, not for use in diagnostic procedures. Expiration Date :
DAB Powder Applications :
Used for IHC and WB Color reaction. Background :
DAB is utilized in many applications for visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide; the oxidized DAB forms an insoluble brown sediment. The solutions of DAB should be made immediately prior to use for the best results. The literature indicates that it is possible to store solutions or chromogen of DAB for several weeks at -20°C. Report indicated DAB dissolved in Tris buffered saline (pH 7.6) at 0.6 mg/ml can be stable for several weeks at 4°C. Alternative Name :
DAB is a possible carcinogen. Operate it with gloves. Classification :
IHC Related Reagents Format :
Powder Contents :
DAB Powder 1g Storage & Shelf Life :
Store at RT. For 12 Months. Procedure :
1. Dissolve 10 mg in 15 ml of PBS or TBS buffer; 2. Add 12 uL of fresh 30% hydrogen peroxide (or 120 mL fresh 3% hydrogen peroxide) prior to use. Research use :
For research use only, not for use in diagnostic procedures. Expiration Date :
Blocking peptide available as BD5031P