MYPT1 (R847) Peptide 
Datasheet
COA
MSDS
SHIP
Product Name :
MYPT1 (R847) Peptide Applications :
Blocking Background :
myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Alternative Name :
PPP1R12A; MBS; MGC133042; MYPT1; Protein phosphatase 1 regulatory subunit 12A Swiss-Prot :
O14974 Product :
1 mg/ml in DI water. Purification&Purity :
Synthetic peptide MYPT1 (R847). (Note: the amino acid sequence is proprietary). The purity is > 98%. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
This peptide can be used with studies using BS1558 MYPT1 (R847) pAb.
MYPT1 (R847) Peptide Applications :
Blocking Background :
myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Alternative Name :
PPP1R12A; MBS; MGC133042; MYPT1; Protein phosphatase 1 regulatory subunit 12A Swiss-Prot :
O14974 Product :
1 mg/ml in DI water. Purification&Purity :
Synthetic peptide MYPT1 (R847). (Note: the amino acid sequence is proprietary). The purity is > 98%. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
This peptide can be used with studies using BS1558 MYPT1 (R847) pAb.
Blocking peptide available as BS1558PP