CstF-50 (R3) Peptide 
Datasheet
COA
MSDS
SHIP
Product Name :
CstF-50 (R3) Peptide Applications :
Blocking Background :
Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits of 77, 64 and 50 kDa. Heterotrimeric CstF recognizes GU and U-rich sequences located downstream of the polyadenylation site on RNA. The 50 kDa CstF subunit shares extensive homology with mammalian G protein beta-subunits and has a transducin repeat domain, which is a 44 amino acid-long sequence that is repeated seven times. CstF-50 interacts with the nuclear protein BARD1 (BRCA1-associated RING domain protein) and inhibits polyadenylation in vitro. CstF-50 may also be responsible for the interaction of the heterotrimeric CstF complex with other polyadenylation and 3'-end cleavage factors to form a stable complex on the pre-mRNA. Alternative Name :
Cleavage stimulation factor subunit 1; CF-1 50 kDa subunit; Cleavage stimulation factor 50 kDa subunit; CSTF 50 kDa subunit; CstF-50; CstF50; CSTF1 Swiss-Prot :
Q05048 Product :
1 mg/ml in DI water. Purification&Purity :
Synthetic peptide CstF-50 (R3). (Note: the amino acid sequence is proprietary). The purity is > 98%. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
This peptide can be used with studies using BS2036 CstF-50 (R3) pAb.
CstF-50 (R3) Peptide Applications :
Blocking Background :
Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits of 77, 64 and 50 kDa. Heterotrimeric CstF recognizes GU and U-rich sequences located downstream of the polyadenylation site on RNA. The 50 kDa CstF subunit shares extensive homology with mammalian G protein beta-subunits and has a transducin repeat domain, which is a 44 amino acid-long sequence that is repeated seven times. CstF-50 interacts with the nuclear protein BARD1 (BRCA1-associated RING domain protein) and inhibits polyadenylation in vitro. CstF-50 may also be responsible for the interaction of the heterotrimeric CstF complex with other polyadenylation and 3'-end cleavage factors to form a stable complex on the pre-mRNA. Alternative Name :
Cleavage stimulation factor subunit 1; CF-1 50 kDa subunit; Cleavage stimulation factor 50 kDa subunit; CSTF 50 kDa subunit; CstF-50; CstF50; CSTF1 Swiss-Prot :
Q05048 Product :
1 mg/ml in DI water. Purification&Purity :
Synthetic peptide CstF-50 (R3). (Note: the amino acid sequence is proprietary). The purity is > 98%. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
This peptide can be used with studies using BS2036 CstF-50 (R3) pAb.
Blocking peptide available as BS2036PP