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Goat Anti-Rabbit IgG (H&L) Highly Cross-Adsorbed - AF594 BS22354
Product NameGoat Anti-Rabbit IgG (H&L) Highly Cross-Adsorbed - AF594
Catalog No.BS22354
Host Goat
ApplicationE, IH, IF, FC
ClassificationSecondary Antibodies
ImmunogenRabbit IgG
LabelAF594
Concentration1 mg/ml
Suggested Dilution RangeIH (1/100 - 1/1000), IF (1/100 - 1/1000), FC (1/1000 - 1/4000), E (Use at an assay dependent concentration)
DescriptionGoat Polyclonal Secondary Antibody to Rabbit IgG (H&L) Highly Cross-Adsorbed and conjugate to AF594
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500ul $540
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Product Name :
Goat Anti-Rabbit IgG (H&L) Highly Cross-Adsorbed - AF594
Host :
Goat
Application :
E, IH, IF, FC
Classification :
Secondary Antibodies
Immunogen :
Rabbit IgG
Label :
AF594
Concentration :
1 mg/ml
Suggested Dilution Range :
IH (1/100 - 1/1000), IF (1/100 - 1/1000), FC (1/1000 - 1/4000), E (Use at an assay dependent concentration)
Description :
Goat Polyclonal Secondary Antibody to Rabbit IgG (H&L) Highly Cross-Adsorbed and conjugate to AF594
Buffer :
1 mg/ml, liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide
Preservative :
0.02% Proclin/Sodium Azide
Purification&Purity :
Goat Polyclonal Secondary Antibody to Rabbit IgG (H&L) have been cross-adsorbed against IgG from bovine, goat, guinea pig, hamster, horse, mouse, rat, and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.
Storage&Stability :
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Expiration Date :
Six months from date of reconstitution.
Background :
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG. No antibody was detected against non immunoglobulin serum proteins.
Blocking peptide available as BS22354P
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