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OCT4 Recombinant Protein NCP0456
Product NameOCT4 Recombinant Protein
Catalog No.NCP0456
Swiss-ProtQ01860
Host E.coli
TagHis-tag;Sumo-Tag
Restriction sitesBamHI-XhoI
Background
Soluble0.5M Urea, PH7.4
Purification&PurityTransferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).
Concentration
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Product Name :
OCT4 Recombinant Protein
Swiss-Prot :
Q01860
Host :
E.coli
Tag :
Amino acid Sequence :
GSMAGHLASDFAFSPPPGGGGDGPGGPEPGWVDPRTWLSFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLVPQGGLETSQPEGEAGVGVESNSDGASPEPCTVTPGAVKLEKEKLEQNPEESQDIKALQKELEQFAKLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSFKNMCKLR-LE
Restriction sites :
BamHI-XhoI
Background :
Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells. A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation. Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker. Oct-4 exists as two splice variants, Oct-4A and Oct-4B. Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells.
Soluble :
0.5M Urea, PH7.4
Purification&Purity :
Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Expression vector :
pSmart-I
BiowMW :
For research use only, not for use in diagnostic procedure.
Note :
500ug/1mg
concentration :
Blocking peptide available as NCP0456P
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