Plasmid name :
pIRES2-EGFP-Flag-GSN Gene/insert name :
GSN Alt name :
ADF; AGEL Insert size :
2349bp Species :
Homo sapiens (human) Gene ID :
2934 Accessions :
NM_000177.5 Vector Backbone :
pIRES2-EGFP Tag :
Flag(N-term);EGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression Backbone size w/o insert :
5300bp Cloning site 5' :
Xho I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens gelsolin with N terminal Flag tag and the unfused EGFP protein at the C-terminus Selectable markers :
Neomycin
pIRES2-EGFP-Flag-GSN Gene/insert name :
GSN Alt name :
ADF; AGEL Insert size :
2349bp Species :
Homo sapiens (human) Gene ID :
2934 Accessions :
NM_000177.5 Vector Backbone :
pIRES2-EGFP Tag :
Flag(N-term);EGFP(C-term) Backbone manufacturer :
Clontech Vector type :
Mammalian Expression Backbone size w/o insert :
5300bp Cloning site 5' :
Xho I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Kanamycin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens gelsolin with N terminal Flag tag and the unfused EGFP protein at the C-terminus Selectable markers :
Neomycin
Blocking peptide available as PPL00880-2bP