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pSpCas9(BB)-2A-GFP-ITGB4 sgRNA (3+1) PPL02117-3
Plasmid namepSpCas9(BB)-2A-GFP-ITGB4 sgRNA (3+1)
Catalog No.PPL02117-3
Gene/insert name
Alt nameCD104; GP150
Insert size20bp
SpeciesHomo sapiens (human)
Gene ID3691
AccessionsN/A
Vector BackbonepSpCas9(BB)-2A-GFP (PX458)
Tag3*Flag(N-term);2A-EGFP(C-term)
Backbone manufacturerN/A
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Plasmid name :
pSpCas9(BB)-2A-GFP-ITGB4 sgRNA (3+1)
Gene/insert name :
ITGB4 sgRNA
Alt name :
CD104; GP150
Insert size :
20bp
Species :
Homo sapiens (human)
Gene ID :
3691
Accessions :
N/A
Vector Backbone :
pSpCas9(BB)-2A-GFP (PX458)
Tag :
3*Flag(N-term);2A-EGFP(C-term)
Backbone manufacturer :
N/A
Vector type :
CRISPR
Backbone size w/o insert :
9288bp
Cloning site 5' :
Bbs I
Site destroyed during cloning :
Yes
Cloning site 3' :
Bbs I
Site destroyed during cloning :
Yes
Bacterial resistance(s) :
Ampicillin
Growth strain(s) :
Top10
Growth temperature :
37°C
High or low copy :
High Copy
Plasmid description :
CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting Homo sapiens integrin subunit beta 4
Selectable markers :
None
Blocking peptide available as PPL02117-3P
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