Plasmid name :
pcDNA3.1(+)-Flag-EGFP-His-TEV-FUS Gene/insert name :
FUS Alt name :
TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2 Insert size :
1581bp Species :
Homo sapiens (human) Gene ID :
2521 Accessions :
NM_004960.4 Vector Backbone :
pcDNA3.1(+) Tag :
Flag(N-term),GFP(N-term),His(N-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
Hind III Site destroyed during cloning :
NO Cloning site 3' :
Not I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens FUS RNA binding protein with the Flag tag, EGFP, His tag, and TEV site at the N-terminus Selectable markers :
Neomycin
pcDNA3.1(+)-Flag-EGFP-His-TEV-FUS Gene/insert name :
FUS Alt name :
TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2 Insert size :
1581bp Species :
Homo sapiens (human) Gene ID :
2521 Accessions :
NM_004960.4 Vector Backbone :
pcDNA3.1(+) Tag :
Flag(N-term),GFP(N-term),His(N-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
Hind III Site destroyed during cloning :
NO Cloning site 3' :
Not I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens FUS RNA binding protein with the Flag tag, EGFP, His tag, and TEV site at the N-terminus Selectable markers :
Neomycin
Blocking peptide available as PPL02039-2aP