Plasmid name :
pSpCas9(BB)-2A-GFP-mTraf3 sgRNA (3+1) Gene/insert name :
Traf3 sgRNA Alt name :
AI528849; amn; CAP-1; CD40bp; CRAF1; LAP1;T-BAM; TRAFAMN Insert size :
20bp Species :
Mus musculus (house mouse) Gene ID :
22031 Accessions :
NM_011632.3 Vector Backbone :
pSpCas9(BB)-2A-GFP (PX458) Tag :
3*Flag(N-term);2A-EGFP(C-term) Backbone manufacturer :
N/A Vector type :
CRISPR Backbone size w/o insert :
9289bp Cloning site 5' :
Bbs I Site destroyed during cloning :
Yes Cloning site 3' :
Bbs I Site destroyed during cloning :
Yes Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting Mus musculus TNF receptor-associated factor 3 Selectable markers :
None

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Blocking peptide available as PPL50125-3P