Plasmid name :
pPLK/GFP+Puro-ANPEP shRNA (3+1) Gene/insert name :
ANPEP shRNA Alt name :
APN; CD13; LAP1; P150; PEPN; GP150 Insert size :
~60bp Species :
Homo sapiens (human) Gene ID :
290 Accessions :
NM_001150 Vector Backbone :
pPLK GFP+Puro Tag :
GFP(N-term) Backbone manufacturer :
N/A Vector type :
Lentiviral interference Backbone size w/o insert :
8000bp Cloning site 5' :
BamH I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
This product consists of three shRNA constructs to ensure effective silencing of the target gene, as well as a scramble shRNA. Selectable markers :
Puromycin
pPLK/GFP+Puro-ANPEP shRNA (3+1) Gene/insert name :
ANPEP shRNA Alt name :
APN; CD13; LAP1; P150; PEPN; GP150 Insert size :
~60bp Species :
Homo sapiens (human) Gene ID :
290 Accessions :
NM_001150 Vector Backbone :
pPLK GFP+Puro Tag :
GFP(N-term) Backbone manufacturer :
N/A Vector type :
Lentiviral interference Backbone size w/o insert :
8000bp Cloning site 5' :
BamH I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
This product consists of three shRNA constructs to ensure effective silencing of the target gene, as well as a scramble shRNA. Selectable markers :
Puromycin
Blocking peptide available as PPL01708-3P