Plasmid name :
pcDNA3.1(+)-Flag-TFEB Gene/insert name :
TFEB Alt name :
TCFEB; BHLHE35; ALPHATFEB Insert size :
1431bp Species :
Homo sapiens (human) Gene ID :
7942 Accessions :
NM_007162.2 Vector Backbone :
pcDNA3.1(+) Tag :
Flag(N-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
BamH I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens transcription factor EB (three point mutation: G63A/G120A/A1164G not causing the amino acid variation) with N terminal Flag tag Selectable markers :
Neomycin
pcDNA3.1(+)-Flag-TFEB Gene/insert name :
TFEB Alt name :
TCFEB; BHLHE35; ALPHATFEB Insert size :
1431bp Species :
Homo sapiens (human) Gene ID :
7942 Accessions :
NM_007162.2 Vector Backbone :
pcDNA3.1(+) Tag :
Flag(N-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
BamH I Site destroyed during cloning :
NO Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens transcription factor EB (three point mutation: G63A/G120A/A1164G not causing the amino acid variation) with N terminal Flag tag Selectable markers :
Neomycin
Blocking peptide available as PPL01283-2bP