Plasmid name :
pLKO.1-TFRC shRNA (3+1) Gene/insert name :
TFRC shRNA Alt name :
CD71; IMD46; p90; T9; TFR; TFR1; TR; TRFR Insert size :
58bp Species :
Homo sapiens (human) Gene ID :
7037 Accessions :
NM_003234 Vector Backbone :
pLKO.1-Puro Tag :
None Backbone manufacturer :
Addgene Vector type :
Lentiviral interference Backbone size w/o insert :
7091bp Cloning site 5' :
Age I Site destroyed during cloning :
YES Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
This product consists of three shRNA constructs to ensure effective silencing of the target gene, as well as a scramble shRNA. Selectable markers :
Puromycin
pLKO.1-TFRC shRNA (3+1) Gene/insert name :
TFRC shRNA Alt name :
CD71; IMD46; p90; T9; TFR; TFR1; TR; TRFR Insert size :
58bp Species :
Homo sapiens (human) Gene ID :
7037 Accessions :
NM_003234 Vector Backbone :
pLKO.1-Puro Tag :
None Backbone manufacturer :
Addgene Vector type :
Lentiviral interference Backbone size w/o insert :
7091bp Cloning site 5' :
Age I Site destroyed during cloning :
YES Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
This product consists of three shRNA constructs to ensure effective silencing of the target gene, as well as a scramble shRNA. Selectable markers :
Puromycin
Blocking peptide available as PPL01778-3P