Plasmid name :
pcDNA3.1(+)-MAP2K3-3*Flag Gene/insert name :
MAP2K3 Alt name :
MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2 Insert size :
1044bp Species :
Homo sapiens (human) Gene ID :
5606 Accessions :
NM_145109 Vector Backbone :
pcDNA3.1(+) Tag :
3*Flag(C-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
Xho I Site destroyed during cloning :
NO Cloning site 3' :
Hind III Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens mitogen-activated protein kinase kinase 3(one point mutation: T360C not causing the amino acid variation) with C terminal 3*Flag tag Selectable markers :
Neomycin
pcDNA3.1(+)-MAP2K3-3*Flag Gene/insert name :
MAP2K3 Alt name :
MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2 Insert size :
1044bp Species :
Homo sapiens (human) Gene ID :
5606 Accessions :
NM_145109 Vector Backbone :
pcDNA3.1(+) Tag :
3*Flag(C-term) Backbone manufacturer :
Invitrogen Vector type :
Mammalian Expression Backbone size w/o insert :
5428bp Cloning site 5' :
Xho I Site destroyed during cloning :
NO Cloning site 3' :
Hind III Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Top10 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
Mammalian Expression vector of Homo sapiens mitogen-activated protein kinase kinase 3(one point mutation: T360C not causing the amino acid variation) with C terminal 3*Flag tag Selectable markers :
Neomycin
Blocking peptide available as PPL01388-2aP