Plasmid name :
pLKO.1-E2F1 shRNA (3+1) Gene/insert name :
E2F1 shRNA Alt name :
RBP3; E2F-1; RBAP1; RBBP3 Insert size :
~50bp Species :
Homo sapiens (human) Gene ID :
1869 Accessions :
N/A Vector Backbone :
pLKO.1-Puro Tag :
None Backbone manufacturer :
Addgene Vector type :
Lentiviral interference Backbone size w/o insert :
7091bp Cloning site 5' :
Xho I Site destroyed during cloning :
YES Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
This product consists of three shRNA constructs to ensure effective silencing of the target gene, as well as a scramble shRNA. Selectable markers :
Puromycin
pLKO.1-E2F1 shRNA (3+1) Gene/insert name :
E2F1 shRNA Alt name :
RBP3; E2F-1; RBAP1; RBBP3 Insert size :
~50bp Species :
Homo sapiens (human) Gene ID :
1869 Accessions :
N/A Vector Backbone :
pLKO.1-Puro Tag :
None Backbone manufacturer :
Addgene Vector type :
Lentiviral interference Backbone size w/o insert :
7091bp Cloning site 5' :
Xho I Site destroyed during cloning :
YES Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
This product consists of three shRNA constructs to ensure effective silencing of the target gene, as well as a scramble shRNA. Selectable markers :
Puromycin
Blocking peptide available as PPL01074-3P